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Literature summary for 3.1.1.101 extracted from

  • Ma, Y.; Yao, M.; Li, B.; Ding, M.; He, B.; Chen, S.; Zhou, X.; Yuan, Y.
    Enhanced poly(ethylene terephthalate) hydrolase activity by protein engineering (2018), Engineering, 4, 888-893 .
No PubMed abstract available
Search for more literature for 3.1.1.101

Application

Application Comment Organism
degradation the enzyme is a potential tool to solve the issue of polyester plastic pollution Ideonella sakaiensis
environmental protection the enzyme is a potential tool to solve the issue of polyester plastic pollution Ideonella sakaiensis

Protein Variants

Protein Variants Comment Organism
I179F enzymatic activity of the mutant enzyme exhibits 2.5fold increase in comparison with wild-type PETase Ideonella sakaiensis
L88F enzymatic activity of the mutant enzyme exhibits 2.1fold increase in comparison with wild-type PETase Ideonella sakaiensis
R61A enzymatic activity of the mutant enzyme exhibits 1.4fold increase in comparison with wild-type PETase Ideonella sakaiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 4-nitrophenyl acetate 30°C, pH 7.0, mutant enzyme I179F Ideonella sakaiensis
3.8
-
 4-nitrophenyl acetate 30°C, pH 7.0, mutant enzyme L88F Ideonella sakaiensis
4.5
-
 4-nitrophenyl acetate 30°C, pH 7.0, mutant enzyme R61A Ideonella sakaiensis
4.6
-
 4-nitrophenyl acetate 30°C, pH 7.0, wild-type enzyme Ideonella sakaiensis

Organism

Organism UniProt Comment Textmining
Ideonella sakaiensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ethylene terephthalate)n + H2O
-
 Ideonella sakaiensis (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
 ?
4-nitrophenyl acetate + H2O
-
 Ideonella sakaiensis 4-nitrophenol + acetate
-
 ?

Synonyms

Synonyms Comment Organism
PETase
-
 Ideonella sakaiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27
-
 4-nitrophenyl acetate 30°C, pH 7.0, wild-type enzyme Ideonella sakaiensis
41.9
-
 4-nitrophenyl acetate 30°C, pH 7.0, mutant enzyme R61A Ideonella sakaiensis
44.8
-
 4-nitrophenyl acetate 30°C, pH 7.0, mutant enzyme L88F Ideonella sakaiensis
107.7
-
 4-nitrophenyl acetate 30°C, pH 7.0, mutant enzyme I179F Ideonella sakaiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.9
-
 4-nitrophenyl acetate 30°C, pH 7.0, wild-type enzyme Ideonella sakaiensis
9.3
-
 4-nitrophenyl acetate 30°C, pH 7.0, mutant enzyme R61A Ideonella sakaiensis
11.8
-
 4-nitrophenyl acetate 30°C, pH 7.0, mutant enzyme L88F Ideonella sakaiensis
89.8
-
 4-nitrophenyl acetate 30°C, pH 7.0, mutant enzyme I179F Ideonella sakaiensis