Literature summary for 3.1.1.101 extracted from

Barth, M.; Honak, A.; Oeser, T.; Wei, R.; Belisario-Ferrari, M.R.; Then, J.; Schmidt, J.; Zimmermann, W.
A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films (2016), Biotechnol. J., 11, 1082-1087 .

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Application

Application	Comment	Organism
degradation	a dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films. Since the enzymatic PET hydrolysis is inhibited by the degradation intermediate 4-[(2-hydroxyethoxy)carbonyl]benzoate, a dual enzyme system consisting of a polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 is employed for the hydrolysis of PET films at 60°C. HPLC analysis of the reaction products obtained after 24 h of hydrolysis shows an increased amount of soluble products with a lower proportion of 4-[(2-hydroxyethoxy)carbonyl]benzoate in the presence of the immobilized carboxylesterase TfCa. The results indicate a continuous hydrolysis of the inhibitory 4-[(2-hydroxyethoxy)carbonyl]benzoate by the immobilized carboxylesterase TfCa and demonstrate its advantage as a second biocatalyst in combination with a polyester hydrolase for an efficient degradation oft PET films	Thermobifida fusca
environmental protection	a dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films. Since the enzymatic PET hydrolysis is inhibited by the degradation intermediate 4-[(2-hydroxyethoxy)carbonyl]benzoate, a dual enzyme system consisting of a polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 is employed for the hydrolysis of PET films at 60°C. HPLC analysis of the reaction products obtained after 24 h of hydrolysis shows an increased amount of soluble products with a lower proportion of 4-[(2-hydroxyethoxy)carbonyl]benzoate in the presence of the immobilized carboxylesterase TfCa. The results indicate a continuous hydrolysis of the inhibitory 4-[(2-hydroxyethoxy)carbonyl]benzoate by the immobilized carboxylesterase TfCa and demonstrate its advantage as a second biocatalyst in combination with a polyester hydrolase for an efficient degradation oft PET films	Thermobifida fusca

Inhibitors

Inhibitors	Comment	Organism	Structure
4-[(2-hydroxyethoxy)carbonyl]benzoate	-	Thermobifida fusca

Organism

Organism	UniProt	Comment	Textmining
Thermobifida fusca	E5BBQ3	-	-
Thermobifida fusca KW3	E5BBQ3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(ethylene terephthalate)n + H2O	-	Thermobifida fusca	(ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate	-	?
(ethylene terephthalate)n + H2O	-	Thermobifida fusca KW3	(ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate	-	?

Synonyms

Synonyms	Comment	Organism
TfCut2	-	Thermobifida fusca

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Release 2023.1 (January 2023)