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Literature summary for 3.1.1.101 extracted from

  • Wei, R.; Oeser, T.; Schmidt, J.; Meier, R.; Barth, M.; Then, J.; Zimmermann, W.
    Engineered bacterial polyester hydrolases efficiently degrade polyethylene terephthalate due to relieved product inhibition (2016), Biotechnol. Bioeng., 113, 1658-1665.
    View publication on PubMed
Search for more literature for 3.1.1.101

Application

Application Comment Organism
degradation exchange of amino acid residues of TfCut2 involved in substrate binding with those present in LC-cutinase, UniProt ID G9BY57, from an uncultured bacterium, leads to enzyme variants with increased PET hydrolytic activity at 65°C. Variant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
G62A exchange of amino acid residues of TfCut2 involved in substrate binding with those present in LC-cutinase, UniProt ID G9BY57, from an uncultured bacterium, leads to enzyme variants with increased PET hydrolytic activity at 65°C. Variant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme. Mutant G62A reveals a 5.5fold lower binding constant to the inhibitor mono-(2-hydroxyethyl) terephthalate than the wild type enzyme Thermobifida fusca
G62A/I213S exchange of amino acid residues of TfCut2 involved in substrate binding with those present in LC-cutinase, UniProt ID G9BY57, from an uncultured bacterium, leads to enzyme variants with increased PET hydrolytic activity at 65°C. Variant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme Thermobifida fusca

Inhibitors

Inhibitors Comment Organism Structure
mono-(2-hydroxyethyl) terephthalate strong inhibition Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca E5BBQ3 cf. EC 3.1.1.74
-
Thermobifida fusca KW3 E5BBQ3 cf. EC 3.1.1.74
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ethylene terephthalate)n + H2O substrates are amorphous PET film or PET fibers Thermobifida fusca (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate terephthalate is the dominant hydrolysis product detected after a reaction time of 50 h at 65°C. Monohydroxyethylene terephthalate which inhibits the hydrolysis of PET is almost completely converted into terephthalate and accounts for less than 9.1% of the total hydrolysis products ?
(ethylene terephthalate)n + H2O substrates are amorphous PET film or PET fibers Thermobifida fusca KW3 (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate terephthalate is the dominant hydrolysis product detected after a reaction time of 50 h at 65°C. Monohydroxyethylene terephthalate which inhibits the hydrolysis of PET is almost completely converted into terephthalate and accounts for less than 9.1% of the total hydrolysis products ?