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Literature summary for 3.1.1.10 extracted from
- Versuche zur Reinigung und Charakterisierung der Atropinesterase aus Kaninchenlebermikrosomen (1974), Hoppe-Seyler's Z. Physiol. Chem., 355, 529-542.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| atropine | competitive inhibition of hydrolysis of butyrylcholine | Oryctolagus cuniculus |  |
| paraoxon | - |
Oryctolagus cuniculus | |
| phosphoric acid-bis-4-nitrophenyl ester | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | 0.017 | atropine | enzyme form Ef VII | Oryctolagus cuniculus | |
| 0.05 | - |
atropine | enzyme form Ef IX | Oryctolagus cuniculus | |
| 1.9 | - |
Butyrylcholine | enzyme form Ef VII | Oryctolagus cuniculus | |
| 16 | - |
Butyrylcholine | enzyme form Ef IX | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
gel filtration | Oryctolagus cuniculus |
| 61000 | - |
1 * 61000, SDS-PAGE | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
5-6 molecular forms | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.706 | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Oryctolagus cuniculus | 4-nitrophenol + acetate | - |
? | |
| atropine + H2O | - |
Oryctolagus cuniculus | tropine + tropate | - |
? | |
| butanilicain + H2O | i.e. 2-butylamino-N-(2-chloro-6-methylphenyl)acetamide | Oryctolagus cuniculus | ? | - |
? | |
| butyrylcholine + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| methylbutyrate + H2O | - |
Oryctolagus cuniculus | methanol + butanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 61000, SDS-PAGE | Oryctolagus cuniculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 68 | - |
atropine | - |
Oryctolagus cuniculus | |
| 1930 | - |
Butyrylcholine | - |
Oryctolagus cuniculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | hydrolysis of methylbutyrate | Oryctolagus cuniculus |
| 7 | 8.5 | hydrolysis of butyrylcholine | Oryctolagus cuniculus |
| 8 | 8.5 | hydrolysis of atropine | Oryctolagus cuniculus |
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