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Literature summary for 3.1.1.1 extracted from
- Design, characterisation and application of alginate-based encapsulated pig liver esterase (2018), J. Biotechnol., 280, 42-48 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | application of encapsulated hydrolases for enzymatic syntheses | Sus scrofa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | encapsulation of pig liver esterase, method development and optimization, overview. The coating of alginate and chitosan beads have been studied by creating various biopolymer hydrogel beads. Enzyme activity and encapsulation efficiency are notably enhanced by chitosan coating of alginate beads. Leaching is caused by the enzymatic reaction acidifying the matrix, which increases enzyme retention through enhanced electrostatic enzyme-alginate interaction but decreased activity through enzyme deactivation. A practical and ready-to-use method for visualising pH in beads during reaction by co-encapsulation of a conventional pH indicator is described including pH control inside the beads that can only be realised by buffering | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Sus scrofa | - |
| liver | - |
Sus scrofa | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| esterase | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
assay at | Sus scrofa |
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