Activating Compound | Comment | Organism | Structure |
---|---|---|---|
n-heptane | the enzyme activity is activated at 10% v/v by 10% | Bacillus sp. SCSIO 15121 | |
n-hexane | the enzyme activity is activated at 5% v/v by 11% | Bacillus sp. SCSIO 15121 | |
Sodium tripolyphosphate | STPP, activates at 0.1% w/v | Bacillus sp. SCSIO 15121 | |
Triton X-100 | activates at 0.1% w/v | Bacillus sp. SCSIO 15121 | |
Tween 20 | activates at 0.1% w/v | Bacillus sp. SCSIO 15121 | |
Tween 80 | activates at 0.1% w/v | Bacillus sp. SCSIO 15121 |
Application | Comment | Organism |
---|---|---|
synthesis | utilization of a marine bacillus esterase in the stereo-selective production of D-methyl lactate through enzymatic kinetic resolution reactions | Bacillus sp. SCSIO 15121 |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged BSE01701 esterase in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Bacillus sp. SCSIO 15121 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 93.5% inhibition at 5 mM | Bacillus sp. SCSIO 15121 | |
Fe2+ | slight inhibition at 1 mM | Bacillus sp. SCSIO 15121 | |
K+ | slight inhibition at 1 mM | Bacillus sp. SCSIO 15121 | |
Mg2+ | slight inhibition at 1 mM | Bacillus sp. SCSIO 15121 | |
Mn2+ | activates at 1 mM, inhibits at 5 mM | Bacillus sp. SCSIO 15121 | |
Ni2+ | almost complete inhibition at 1-5 mM | Bacillus sp. SCSIO 15121 | |
Zn2+ | complete inhibition at 1-5 mM | Bacillus sp. SCSIO 15121 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus sp. SCSIO 15121 | |
0.5 | - |
4-nitrophenyl butyrate | pH 8.5, 35°C, recombinant enzyme | Bacillus sp. SCSIO 15121 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at 1-5 mM | Bacillus sp. SCSIO 15121 | |
Mn2+ | activates at 1 mM, inhibits at 5 mM | Bacillus sp. SCSIO 15121 |
Organic Solvent | Comment | Organism |
---|---|---|
1,4-dioxane | the enzyme activity is reduced by 60-95.5% at 5-10% v/v | Bacillus sp. SCSIO 15121 |
Acetone | the enzyme activity is reduced by 44% at 10% v/v | Bacillus sp. SCSIO 15121 |
dichloromethane | the enzyme activity is reduced by 73-91% at 5-10% v/v | Bacillus sp. SCSIO 15121 |
DMSO | the enzyme activity is stable at 5-10% v/v | Bacillus sp. SCSIO 15121 |
Methanol | the enzyme activity is reduced by 38% at 10% v/v | Bacillus sp. SCSIO 15121 |
n-decane | the enzyme activity is reduced by 11-23% at 5-10% v/v | Bacillus sp. SCSIO 15121 |
n-heptane | the enzyme activity is stable at 5-10% v/v | Bacillus sp. SCSIO 15121 |
n-hexane | the enzyme activity is stable at 5-10% v/v | Bacillus sp. SCSIO 15121 |
n-octanol | the enzyme activity is reduced by 22-90% at 5-10% v/v | Bacillus sp. SCSIO 15121 |
n-propanol | the enzyme activity is reduced by 93.5% at 10% v/v | Bacillus sp. SCSIO 15121 |
tetrahydrofuran | the enzyme activity is reduced by 33-96% at 5-10% v/v | Bacillus sp. SCSIO 15121 |
toluene | the enzyme activity is reduced by 46.5-62% at 5-10% v/v | Bacillus sp. SCSIO 15121 |
xylene | the enzyme activity is reduced by 44% at 5-10% v/v | Bacillus sp. SCSIO 15121 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. SCSIO 15121 | A0A1B2BII0 | isolated from the Indian Ocean | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged BSE01701 esterase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Bacillus sp. SCSIO 15121 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | high activity | Bacillus sp. SCSIO 15121 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | best 4-nitrophenyl ester substrate | Bacillus sp. SCSIO 15121 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl hexanoate + H2O | lower activity | Bacillus sp. SCSIO 15121 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | low activity | Bacillus sp. SCSIO 15121 | 4-nitrophenol + octanoate | - |
? | |
additional information | esterase BSE01701 can enzymatically resolve inexpensive racemic methyl lactate and generate chiral D-methyl lactate, BSE01701 is utilized to generate optically pure D-methyl lactate through enzymatic kinetic resolution reactions, optimum conditions are 35°C, pH 9.0, and 60 % n-heptane. Enzyme BSE01701 preferentially hydrolyzes 4-nitrophenyl esters with short-chain fatty acids and exhibits its highest activity toward 4-nitrophenyl butyrate. The hydrolysis activities of BSE01701 are much lower when the aliphatic acyl-chain of substrate is longer than C8. BSE01701 is an esterase | Bacillus sp. SCSIO 15121 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28973, sequence calculation | Bacillus sp. SCSIO 15121 |
Synonyms | Comment | Organism |
---|---|---|
Bacillus esterase | - |
Bacillus sp. SCSIO 15121 |
BSE01701 | - |
Bacillus sp. SCSIO 15121 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Bacillus sp. SCSIO 15121 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | over 60% of maximal activity within this range | Bacillus sp. SCSIO 15121 |
30 | 45 | over 80% of maximal activity within this range | Bacillus sp. SCSIO 15121 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
purified recombinant His-tagged enzyme, 12 h, loss of 40% activity | Bacillus sp. SCSIO 15121 |
25 | - |
purified recombinant His-tagged enzyme, 12 h, loss of 60% activity | Bacillus sp. SCSIO 15121 |
35 | - |
purified recombinant His-tagged enzyme, 12 h, loss of 70% activity | Bacillus sp. SCSIO 15121 |
45 | - |
purified recombinant His-tagged enzyme, 2 h, loss of over 95% activity, after 4 h inactivation | Bacillus sp. SCSIO 15121 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1044 | - |
4-nitrophenyl butyrate | pH 8.5, 35°C, recombinant enzyme | Bacillus sp. SCSIO 15121 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus sp. SCSIO 15121 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | high activity within this range | Bacillus sp. SCSIO 15121 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | purified recombinant His-tagged enzyme, 35°C, over 60% activity retained after 12 h, a sharp decrease in hydrolysis activity is observed at over pH 9.0 | Bacillus sp. SCSIO 15121 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. SCSIO 15121 | sequence calculation | - |
5.62 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2088 | - |
4-nitrophenyl butyrate | pH 8.5, 35°C, recombinant enzyme | Bacillus sp. SCSIO 15121 |