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Literature summary for 3.1.1.1 extracted from

  • Almeida, R.; Alqueres, S.; Larentis, A.; Rössle, S.; Cardoso, A.; Almeida, W.; Bisch, P.; Alves, T.; Martins, O.
    Cloning, expression, partial characterization and structural modeling of a novel esterase from Pyrococcus furiosus (2006), Enzyme Microb. Technol., 39, 1128-1136 .
No PubMed abstract available
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
PMSF completely inhibited by 1 mM Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8TZJ1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
 Pyrococcus furiosus 4-methylumbelliferol + acetate
-
 ?
4-methylumbelliferyl heptanoate + H2O
-
 Pyrococcus furiosus 4-methylumbelliferol + heptanoate
-
 ?
4-methylumbelliferyl palmitate + H2O
-
 Pyrococcus furiosus 4-methylumbelliferol + palmitate
-
 ?

Synonyms

Synonyms Comment Organism
PF2001
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 50% of maximal activity, 70°C: about 65% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 thermostable for 120 min Pyrococcus furiosus
100
-
 10 min, complete loss of activity Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: 71% of maximal activity, pH 8.0: 82% of maximal activity Pyrococcus furiosus