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Literature summary for 3.1.1.1 extracted from

  • Shao, H.; Xu, L.; Yan, Y.
    Biochemical characterization of a carboxylesterase from the archaeon Pyrobaculum sp. 1860 and a rational explanation of its substrate specificity and thermostability (2014), Int. J. Mol. Sci., 15, 16885-16910.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
functionally expressed in Escherichia coli as His-tagged protein Pyrobaculum sp.

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile 15% v/v, 1.8fold activation. 30% v/v, 32% loss of activity Pyrobaculum sp.
Co2+ 1 mM, 5% inhibition. 10 mM, 27% inhibition Pyrobaculum sp.
Cu2+ 1 mM, 15% inhibition. 10 mM, 95% inhibition Pyrobaculum sp.
DMSO 30% v/v, 22% loss of activity Pyrobaculum sp.
ethanol 15% v/v, 1.7fold activation. 30% v/v, 18% loss of activity Pyrobaculum sp.
Isopropanol 15% v/v, 1.4fold activation. 30% v/v, 21% loss of activity Pyrobaculum sp.
Mg2+ 1 mM, 5% loss of activity Pyrobaculum sp.
Mn2+ 1 mM; 10 mM, 12% loss of activity Pyrobaculum sp.
PMSF 1 mM, 28% loss of activity Pyrobaculum sp.
SDS 0.1% v/v, 17% loss of activity. 1% v/v, 68% loss of activity Pyrobaculum sp.
Tween-20 0.1% v/v, 16% loss of activity. 1% v/v, 33% loss of activity Pyrobaculum sp.
Zn2+ 1 mM, 8% inhibition. 10 mM, 49% inhibition Pyrobaculum sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
4-nitrophenyl acetate pH 8.0, 80°C Pyrobaculum sp.
1.27
-
4-nitrophenyl butyrate pH 8.0, 80°C Pyrobaculum sp.
2.64
-
4-nitrophenyl octanoate pH 8.0, 80°C Pyrobaculum sp.
4.99
-
4-nitrophenyl decanoate pH 8.0, 80°C Pyrobaculum sp.

Metals/Ions

Metals/Ions Comment Organism Structure
acetone 15% or 30% v/v, 1.2fold activation Pyrobaculum sp.
acetonitrile 15% v/v, 1.8fold activation. 30% v/v, 32% loss of activity Pyrobaculum sp.
Ca2+ 10 mM, 10 mM, 1.1fold activation Pyrobaculum sp.
CTAB 0.1% v/v, 1.4fold activation. 1% v/v, 1.8fold activation Pyrobaculum sp.
ethanol 15% v/v, 1.7fold activation. 30% v/v, 18% loss of activity Pyrobaculum sp.
Isopropanol 15% v/v, 1.4fold activation. 30% v/v, 21% loss of activity Pyrobaculum sp.
K+ 10 mM, 1.2fold activation Pyrobaculum sp.
methanol 15% v/v, 1.4fold activation. 30% v/v, 1.7fold activation Pyrobaculum sp.
Mg2+ 10 mM, 1.2fold activation Pyrobaculum sp.
Na+ 10 mM, 1.1fold activation Pyrobaculum sp.
Triton X-100 0.1% v/v, 1.3fold activation. 1% v/v, 31% loss of activity Pyrobaculum sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21130
-
calculated from sequence Pyrobaculum sp.

Organism

Organism UniProt Comment Textmining
Pyrobaculum sp. G7VG08
-
-
Pyrobaculum sp. 1860 G7VG08
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrobaculum sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Pyrobaculum sp. 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Pyrobaculum sp. 1860 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Pyrobaculum sp. 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Pyrobaculum sp. 1860 4-nitrophenol + butyrate
-
?
4-nitrophenyl decanoate + H2O
-
Pyrobaculum sp. 4-nitrophenol + decanoate
-
?
4-nitrophenyl decanoate + H2O
-
Pyrobaculum sp. 1860 4-nitrophenol + decanoate
-
?
4-nitrophenyl octanoate + H2O
-
Pyrobaculum sp. 4-nitrophenol + octanoate
-
?
4-nitrophenyl octanoate + H2O
-
Pyrobaculum sp. 1860 4-nitrophenol + octanoate
-
?

Synonyms

Synonyms Comment Organism
P186_1588
-
Pyrobaculum sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Pyrobaculum sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 40°C: about 50% of maximal activity, 90°C: 80% of maximal activity Pyrobaculum sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
circular dichroism results indicated that rP186_1588 shows slight structure alteration from 60 to 90 °C. Comparative molecular simulations at different temperatures (27°C, 80°C, 100°C and 200°C) reveal that its thermostability is associated with its conformational rigidity Pyrobaculum sp.
90
-
6 h, the enzyme retains 70% of relative activity Pyrobaculum sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.19
-
4-nitrophenyl decanoate pH 8.0, 80°C Pyrobaculum sp.
6.36
-
4-nitrophenyl octanoate pH 8.0, 80°C Pyrobaculum sp.
9.12
-
4-nitrophenyl butyrate pH 8.0, 80°C Pyrobaculum sp.
11.65
-
4-nitrophenyl acetate pH 8.0, 80°C Pyrobaculum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pyrobaculum sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7.0: about 50% of maxiomal activity, pH 10.0: about 60% of maximal activity Pyrobaculum sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pyrobaculum sp. calculated
-
6.3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.24
-
4-nitrophenyl decanoate pH 8.0, 80°C Pyrobaculum sp.
2.41
-
4-nitrophenyl octanoate pH 8.0, 80°C Pyrobaculum sp.
7.18
-
4-nitrophenyl butyrate pH 8.0, 80°C Pyrobaculum sp.
33.29
-
4-nitrophenyl acetate pH 8.0, 80°C Pyrobaculum sp.