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Literature summary for 3.1.1.1 extracted from

  • Nam, J.-K.; Park, Y.-J.; Lee, H.-B.
    Cloning, expression, purification, and characterization of a thermostable esterase from the archaeon Sulfolobus solfataricus P1 (2013), J. Mol. Catal. B, 94, 95-103.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
D266N specific activity of the mutant enzyme is 2% of the wild-type activity Saccharolobus solfataricus
H295N specific activity of the mutant enzyme is 2% of the wild-type activity Saccharolobus solfataricus
S144A specific activity of the mutant enzyme is 1% of the wild-type activity Saccharolobus solfataricus
S144C specific activity of the mutant enzyme is 5% of the wild-type activity Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
diethylfluorophosphate 0.01 mM, 30°C, 30 min, 75% inhibition Saccharolobus solfataricus
diphenylamine-2-carboxylate 5 mM, 30°C, 30 min, 77% inhibition Saccharolobus solfataricus
eserine 5 mM, 30°C, 30 min, 18% inhibition Saccharolobus solfataricus
paraoxon 5 mM, 30°C, 30 min, 90% inhibition Saccharolobus solfataricus
PCMB 5 mM, 30°C, 30 min, 72% inhibition Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
4-nitrophenyl decanoate pH 8.0, 60°C Saccharolobus solfataricus
0.029
-
4-nitrophenyl octanoate pH 8.0, 60°C Saccharolobus solfataricus
0.037
-
4-nitrophenyl dodecanoate pH 8.0, 60°C Saccharolobus solfataricus
0.051
-
4-nitrophenyl hexanoate pH 8.0, 60°C Saccharolobus solfataricus
0.13
-
4-nitrophenyl hexadecanoate pH 8.0, 60°C Saccharolobus solfataricus
0.222
-
4-nitrophenyl butyrate pH 8.0, 60°C Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require a divalent cation for activity Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34260
-
1 * 34260, calculated from sequence Saccharolobus solfataricus
35000
-
gel filtration Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol 90%, 30°C, 30 min, stable Saccharolobus solfataricus
Ethanol 90%, 30°C, 30 min, stable Saccharolobus solfataricus
Methanol 90%, 30°C, 30 min, stable Saccharolobus solfataricus
SDS 5%, 30°C, 30 min, about 70% loss of activity Saccharolobus solfataricus
urea 8 M, 30°C, 30 min, about 80% loss of activity Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus M5DDN2
-
-
Saccharolobus solfataricus P1 M5DDN2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1392
-
pH 8.0, 60°C, purified enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
Saccharolobus solfataricus 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Saccharolobus solfataricus P1 4-nitrophenol + butyrate
-
?
4-nitrophenyl decanoate + H2O
-
Saccharolobus solfataricus 4-nitrophenol + decanoate
-
?
4-nitrophenyl decanoate + H2O
-
Saccharolobus solfataricus P1 4-nitrophenol + decanoate
-
?
4-nitrophenyl dodecanoate + H2O
-
Saccharolobus solfataricus 4-nitrophenol + dodecanoate
-
?
4-nitrophenyl dodecanoate + H2O
-
Saccharolobus solfataricus P1 4-nitrophenol + dodecanoate
-
?
4-nitrophenyl hexadecanoate + H2O
-
Saccharolobus solfataricus 4-nitrophenol + palmitate
-
?
4-nitrophenyl hexanoate + H2O
-
Saccharolobus solfataricus 4-nitrophenol + hexanoate
-
?
4-nitrophenyl hexanoate + H2O
-
Saccharolobus solfataricus P1 4-nitrophenol + hexanoate
-
?
4-nitrophenyl octanoate + H2O
-
Saccharolobus solfataricus 4-nitrophenol + octanoate
-
?
4-nitrophenyl octanoate + H2O
-
Saccharolobus solfataricus P1 4-nitrophenol + octanoate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Saccharolobus solfataricus
monomer 1 * 34260, calculated from sequence Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 90 70°C: about 55% of maximal activity, 90°C: about 80% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
120 h, stable Saccharolobus solfataricus
80
-
120 h, 70% loss of activity Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
861
-
4-nitrophenyl butyrate pH 8.0, 60°C Saccharolobus solfataricus
1138
-
4-nitrophenyl hexadecanoate pH 8.0, 60°C Saccharolobus solfataricus
1312
-
4-nitrophenyl dodecanoate pH 8.0, 60°C Saccharolobus solfataricus
1476
-
4-nitrophenyl hexanoate pH 8.0, 60°C Saccharolobus solfataricus
1491
-
4-nitrophenyl octanoate pH 8.0, 60°C Saccharolobus solfataricus
2337
-
4-nitrophenyl decanoate pH 8.0, 60°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 40% of maximal activity, pH 9.0: about 50% of maximal activity Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3900
-
4-nitrophenyl butyrate pH 8.0, 60°C Saccharolobus solfataricus
8700
-
4-nitrophenyl hexadecanoate pH 8.0, 60°C Saccharolobus solfataricus
29100
-
4-nitrophenyl hexanoate pH 8.0, 60°C Saccharolobus solfataricus
35900
-
4-nitrophenyl dodecanoate pH 8.0, 60°C Saccharolobus solfataricus
51800
-
4-nitrophenyl octanoate pH 8.0, 60°C Saccharolobus solfataricus
97400
-
4-nitrophenyl decanoate pH 8.0, 60°C Saccharolobus solfataricus