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Literature summary for 3.1.1.1 extracted from

  • Wang, Y.; Li, B.; Han, W.; Yang, G.; Zhang, Z.; Feng, Y.
    Redesigning the active site of a carboxyl esterase from the archaeon Archaeoglobus fulgidus to improve sensitivity to organophosphorus compounds (2012), Process Biochem., 47, 2219-2226.
No PubMed abstract available

Application

Application Comment Organism
analysis wild-type enzyme and mutant enzyme N44S/S48V have the potential to be a biosensor for pesticide detection Archaeoglobus fulgidus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
N44S the sensitivity of the mutant enzyme for the large-sized inhibitor profenofos is 1.3fold of that observed for wild-type enzyme, but for the other inhibitors, the sensitivity decreases Archaeoglobus fulgidus
N44S/S48V 1.5fold increase in esterase catalytic activity on 4-nitrophenyl dodecanoate. The sensitivity for the large-sized inhibitors chlorpyrifos, diazinon, triazophos, phoxim, and profenofos increases, which are 2.5-, 2.5-, 4.6-, 3.9-, and 3.1fold of that observed for wild-type AFEST, respectively Archaeoglobus fulgidus
S48V the sensitivity of the mutant enzyme for the smallest inhibitor dichlorvos and medium-sized inhibitor phoxim is 1.6-, and 1.3fold of that observed for wild-type enzyme, respectively and has no obvious changes in the sensitivity towards paraoxon and large inhibitors (e.g., chlorpyrifos, triazophos, and profenofos). The IC50 of S48V for the large inhibitor diazinon is 1.9fold of that observed for wild-type enzyme Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
diazinon
-
Archaeoglobus fulgidus
dichlorvos
-
Archaeoglobus fulgidus
paraoxon
-
Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28558
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl dodecanoate + H2O
-
Archaeoglobus fulgidus 4-nitrophenol + dodecanoate
-
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Synonyms

Synonyms Comment Organism
AF1716
-
Archaeoglobus fulgidus
AFEST
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
half-life for wild-type enzyme: 17 d, half-life for mutant enzyme N44S/S48V: 15 d Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
assay at Archaeoglobus fulgidus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000059
-
pH 7.1, 50°C Archaeoglobus fulgidus paraoxon
0.0000508
-
pH 7.1, 50°C Archaeoglobus fulgidus dichlorvos
0.0075
-
pH 7.1, 50°C Archaeoglobus fulgidus diazinon