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Literature summary for 3.1.1.1 extracted from

  • Manco, G.; Giosue, E.; DAuria, S.; Herman, P.; Carrea, G.; Rossi, M.
    Cloning, overexpression, and properties of a new thermophilic and thermostable esterase with sequence similarity to hormone-sensitive lipase subfamily from the archaeon Archaeoglobus fulgidus (2000), Arch. Biochem. Biophys., 373, 182-192.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression system in Escherichia coli Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic)acid
-
Archaeoglobus fulgidus
diethyldicarbonate 1.5 mM, 94% inhibition Archaeoglobus fulgidus
diisopropyl fluorophosphate irreversible Archaeoglobus fulgidus
phenylmethylsulfonyl fluoride 0.08 mM, 97% inhibition Archaeoglobus fulgidus
physostigmine 0.1 mM, 96% inhibition Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
4-nitrophenyl hexanoate 70°C, pH 7.1 Archaeoglobus fulgidus
0.02
-
4-nitrophenyl pentanoate 70°C, pH 7.1 Archaeoglobus fulgidus
0.021
-
4-nitrophenyl butyrate 70°C, pH 7.1 Archaeoglobus fulgidus
0.106
-
4-nitrophenyl propionate 70°C, pH 7.1 Archaeoglobus fulgidus
0.119
-
4-nitrophenyl octanoate 70°C, pH 7.1 Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33800
-
1 * 33800, SDS-PAGE Archaeoglobus fulgidus
43000
-
gel filtration Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28558
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3000
-
80°C, pH 7.1, substrate: 4-nitrophenyl hexanoate Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
Archaeoglobus fulgidus 4-nitrophenol + butyrate
-
?
4-nitrophenyl hexanoate + H2O
-
Archaeoglobus fulgidus 4-nitrophenol + hexanoate
-
?
4-nitrophenyl octanoate + H2O
-
Archaeoglobus fulgidus 4-nitrophenol + octanoate
-
?
4-nitrophenyl pentanoate + H2O
-
Archaeoglobus fulgidus 4-nitrophenol + pentanoate
-
?
4-nitrophenyl propionate + H2O
-
Archaeoglobus fulgidus 4-nitrophenol + propionate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 33800, SDS-PAGE Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
AFEST
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 100 50°C: about 50% of maximal activity, 100°C: about 90% of maximal activity Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
t1/2: 30 h Archaeoglobus fulgidus
70
-
t1/2: 7.5 h Archaeoglobus fulgidus
85
-
t1/2: 60 min Archaeoglobus fulgidus
90
-
t1/2: 28 min Archaeoglobus fulgidus
95
-
t1/2: 26 min Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
348
-
4-nitrophenyl propionate 70°C, pH 7.1 Archaeoglobus fulgidus
680
-
4-nitrophenyl butyrate 70°C, pH 7.1 Archaeoglobus fulgidus
770
-
4-nitrophenyl octanoate 70°C, pH 7.1 Archaeoglobus fulgidus
866
-
4-nitrophenyl pentanoate 70°C, pH 7.1 Archaeoglobus fulgidus
1014
-
4-nitrophenyl hexanoate 70°C, pH 7.1 Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
Archaeoglobus fulgidus
7.1
-
assay at Archaeoglobus fulgidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3300
-
4-nitrophenyl propionate 70°C, pH 7.1 Archaeoglobus fulgidus
6500
-
4-nitrophenyl octanoate 70°C, pH 7.1 Archaeoglobus fulgidus
32400
-
4-nitrophenyl butyrate 70°C, pH 7.1 Archaeoglobus fulgidus
43300
-
4-nitrophenyl pentanoate 70°C, pH 7.1 Archaeoglobus fulgidus
92200
-
4-nitrophenyl hexanoate 70°C, pH 7.1 Archaeoglobus fulgidus