Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cephaloridine | competitive inhibitor of 4-nitrophenyl butyrate | uncultured bacterium | |
cephalothin | competitive inhibitor of 4-nitrophenyl butyrate | uncultured bacterium | |
additional information | EstU1 activity is not affected by the presence of EDTA (94% activity at 1 mM) | uncultured bacterium | |
phenylmethylsulfonyl fluoride | EstU1 loses approximately 70% of its activity in the presence of 1 mM phenylmethylsulfonyl fluoride | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00603 | - |
4-nitrophenyl butyrate | at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium | |
0.07849 | - |
cefazolin | at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | EstU1 activity is not affected by the presence of Ca2+ (91% activity at 1 mM), Co2+ (89% activity at 1 mM), Cu2+ (88% activity at 1 mM), Fe2+ (84% activity at 1 mM), Mg2+ (91% activity at 1 mM), Mn2+ (74% activity at 1 mM), Ni2+ (89% activity at 1 mM), and Zn2+ (78% activity at 1 mM) | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity resin column chromatography and Superdex-75 gel filtration | uncultured bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.75 | - |
using 4-nitrophenyl decanoate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium |
6.24 | - |
using 4-nitrophenyl acetate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium |
7.46 | - |
using 4-nitrophenyl octanoate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium |
11.78 | - |
using 4-nitrophenyl hexanoate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium |
22.24 | - |
using 4-nitrophenyl butyrate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
uncultured bacterium | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | highest activity | uncultured bacterium | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
uncultured bacterium | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
uncultured bacterium | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
uncultured bacterium | 4-nitrophenol + octanoate | - |
? | |
ampicillin + H2O | - |
uncultured bacterium | ? | - |
? | |
cefazolin + H2O | - |
uncultured bacterium | (6R,7R)-7-amino-3-{[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl}-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (1H-tetrazol-1-yl)acetic acid | - |
? | |
cephaloridine + H2O | - |
uncultured bacterium | (6R,7R)-7-amino-8-oxo-3-[(pyridin-1-ium-1-yl)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate + (thiophen-2-yl)acetic acid | - |
? | |
cephalothin + H2O | - |
uncultured bacterium | acetate + deacetylcephalothin | - |
? | |
additional information | no enzyme activity is detected toward 4-nitrophenyl laurate, 4-nitrophenyl myristate, 4-nitrophenyl palmitate, and 4-nitrophenyl stearate | uncultured bacterium | ? | - |
? | |
nitrocefin + H2O | i.e. 3-(2,4-dinitrosyl)-(6R,7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic acid | uncultured bacterium | ? | - |
? | |
penicillin G + H2O | - |
uncultured bacterium | 6-aminopenicillanic acid + phenylacetic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
EstU1 | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
uncultured bacterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 75 | - |
uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000238 | - |
cefazolin | at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium | |
15.72 | - |
4-nitrophenyl butyrate | at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
uncultured bacterium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
cephalothin | at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium | |
0.215 | - |
cephaloridine | at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003035 | - |
cefazolin | at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium | |
2608 | - |
4-nitrophenyl butyrate | at 35°C in 100 mM sodium phosphate (pH 7.0) | uncultured bacterium |