Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
D205A | mutant shows decreased activity compared to the wild type enzyme | Acetivibrio thermocellus |
D205N | mutant shows decreased activity compared to the wild type enzyme | Acetivibrio thermocellus |
H208A | mutant displays no significant catalytic activity | Acetivibrio thermocellus |
S44A | mutant displays no significant catalytic activity | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
4-nitrophenyl acetate | mutant enzyme D205N | Acetivibrio thermocellus | |
1.8 | - |
4-nitrophenyl acetate | mutant enzyme D205A | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + acetate | - |
? | |
alpha-naphthyl acetate + H2O | - |
Acetivibrio thermocellus | alpha-naphthol + acetate | - |
? | |
additional information | the enzyme does not attack cephalosporin C, N-acetyl-glucosamine, acetyl deoxyadenosine, aryl-ferulates and aryl-coumorates | Acetivibrio thermocellus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Acetyl esterase | - |
Acetivibrio thermocellus |
Ces3-1 | - |
Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 74 | isozymr Ces3-1 retains maximum catalytic activity when incubated for 15 min up to 70°C, after which the esterase is rapidly inactivated displaying no activity after treatment at 74°C for 15 min | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.175 | - |
4-nitrophenyl acetate | mutant enzyme D205N | Acetivibrio thermocellus | |
0.665 | - |
4-nitrophenyl acetate | mutant enzyme D205A | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Acetivibrio thermocellus |