Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
ketoprofen ethyl ester | - |
Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
gel filtration | Archaeoglobus fulgidus |
Organic Solvent | Comment | Organism |
---|---|---|
Triton X-100 | 960% increase of activity in the presence of Triton X-100 | Archaeoglobus fulgidus |
Triton X-15 | 110% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
Triton X-165 | 1649% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
Triton X-305 | 1528% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
Triton X-45 | 562% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
Tween | more than 1200% increase of activity in the presence of Tween 20, 40, 60 or 80 | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
strain DSM 4304 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | highest activity | Archaeoglobus fulgidus | 4-nitrophenol + butyrate | - |
? | |
ketoprofen ethyl ester + H2O | - |
Archaeoglobus fulgidus | ketoprofen + ethanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27500, gel filtration | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
Est-AF | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
using ketoprofen ethyl ester as substrate | Archaeoglobus fulgidus |
80 | - |
using 4-nitrophenyl butyrate as substrate | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 90 | more than 90% activity remains at 65°C or 70°C, 10% of activity remains after 3 h incubation at 90°C using ketoprofen ethyl ester as substrate | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Archaeoglobus fulgidus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | enzyme activity decreases above pH 8.0, no enzyme activity is observed at pH 3.0 and 12.0 | Archaeoglobus fulgidus |