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Literature summary for 3.1.1.1 extracted from

  • Cordova, J.; Ryan, J.D.; Boonyaratanakornkit, B.B.; Clark, D.S.
    Esterase activity of bovine serum albumin up to 160 °C: A new benchmark for biocatalysis (2008), Enzyme Microb. Technol., 42, 278-283.
No PubMed abstract available
Search for more literature for 3.1.1.1

General Stability

General Stability Organism
esterase activity diminishes to 50% upon disruption of the protein's disulfide bridges and disappears completely when digested by proteases Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 4-nitrophenyl palmitate at 70°C Bos taurus
0.0191
-
 4-nitrophenyl palmitate at 150°C Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
SDS for solutions containing 0.05 mM substrate, the optimal SDS concentration is 2.8 mM, and increasing the SDS concentration to 22 mM leads to complete inhibition of activity, solutions containing 0.5 mM substrate display optimal activity in 5.6 mM SDS and near complete inhibition observed in 65 mM SDS Bos taurus
urea bovine serum albumin esterase activity is retained in 8 M urea Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl butyrate + H2O
-
 Bos taurus 4-methylumbelliferol + butyrate
-
 ?
4-methylumbelliferyl oleate + H2O
-
 Bos taurus 4-methylumbelliferol + oleate
-
 ?
4-nitrophenyl acetate + H2O
-
 Bos taurus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Bos taurus 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caprate + H2O
-
 Bos taurus 4-nitrophenol + caprate
-
 ?
4-nitrophenyl caproate + H2O
-
 Bos taurus 4-nitrophenol + caproate
-
 ?
4-nitrophenyl caprylate + H2O highest activity Bos taurus 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl laurate + H2O
-
 Bos taurus 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O
-
 Bos taurus 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Bos taurus 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl propionate + H2O
-
 Bos taurus 4-nitrophenol + propionate
-
 ?
additional information triglycerides are not hydrolyzed Bos taurus ?
-
 ?
palmitin + H2O
-
 Bos taurus ?
-
 ?
Tween 20 + H2O
-
 Bos taurus ?
-
 ?
Tween 80 + H2O
-
 Bos taurus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 160 bovine serum albumin exhibits a 133fold increase in its turnover number toward 4-nitrophenyl palmitate from 70 to 150°C, at 160°C a slight decrease in esterase activity is observed Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00318
-
 4-nitrophenyl palmitate at 70°C Bos taurus
0.424
-
 4-nitrophenyl palmitate at 150°C Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Bos taurus