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Literature summary for 3.1.1.1 extracted from

  • Pena-Montes, C.; Gonzalez, A.; Castro-Ochoa, D.; Farres, A.
    Purification and biochemical characterization of a broad substrate specificity thermostable alkaline protease from Aspergillus nidulans (2008), Appl. Microbiol. Biotechnol., 78, 603-612.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 10 mM Cu2+ reduces the esterase activity by 65% Aspergillus nidulans
Fe2+ inhibition at 10 mM Aspergillus nidulans
Fe3+ inhibition at 10 mM Aspergillus nidulans
Hg2+ inhibition at 1 and 10 mM Aspergillus nidulans
additional information EDTA, Mg2+, Mn2+, Ca2+, and Ba2+ do not affect the esterase activity, the enzyme is not sensitive to the addition of 1 mM SDS Aspergillus nidulans
Phenylmethylsulphonyl fluoride inhibits at 10 mM Aspergillus nidulans
Zn2+ inhibition at 10 mM Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM Co2+ increases activity up to 156% Aspergillus nidulans
Fe2+ up to 238% activation of the enzyme is observed in the presence of 1 mM Fe2+ Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
SDS-PAGE Aspergillus nidulans

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 30% (v/v) acetone decreases the activity by 40%, 50% acetone results in a severe reduction of activity Aspergillus nidulans
dimethylsulfoxide 30% (v/v) dimethylsulfoxide decreases the activity by 40%, 50% (v/v) dimethylsulfoxide results in a severe reduction of activity Aspergillus nidulans
Ethanol 30% (v/v) ethanol increases the esterase activity by 20% Aspergillus nidulans
isopropyl ether 15% (v/v) isopropyl ether increases the esterase activity by about 60%, 50% (v/v) isopropyl ether produces a 10% increase in esterase activity Aspergillus nidulans
Methanol 30% (v/v) methanol increases the esterase activity by 10% Aspergillus nidulans
SDS the denaturing effect of SDS is noticeable at 10 mM Aspergillus nidulans
Spa-80 decreases activity at 0.1% (v/v) and 1% (v/v) Aspergillus nidulans
taurocholic acid slightly increases activity at 0.1% (v/v) Aspergillus nidulans
Triton X-100 decreases activity at 0.1% (v/v) and 1% (v/v) Aspergillus nidulans
Tween 0.1% Tween-80 increases activity by 160%, in the presence of 1% detergent, Tween-80 increases activity by 126% Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-
Aspergillus nidulans PW1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ultrogel ACA-54 gel filtration and HPLC chromatography Aspergillus nidulans

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.113
-
crude extract, at 25°C, pH 7.2 Aspergillus nidulans
5.62
-
after 49.73fold purification, at 25°C, pH 7.2 Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl laurate + H2O
-
Aspergillus nidulans 2-nitrophenol + laurate
-
?
4-nitrophenyl acetate + H2O
-
Aspergillus nidulans 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Aspergillus nidulans PW1 4-nitrophenol + acetate
-
?
4-nitrophenyl laurate + H2O
-
Aspergillus nidulans 4-nitrophenol + laurate
-
?
alpha-naphthyl acetate + H2O
-
Aspergillus nidulans alpha-naphthol + acetate
-
?
alpha-naphthyl acetate + H2O
-
Aspergillus nidulans PW1 alpha-naphthol + acetate
-
?
dicaprin + H2O
-
Aspergillus nidulans ?
-
?
diolein + H2O
-
Aspergillus nidulans ?
-
?
diolein + H2O
-
Aspergillus nidulans PW1 ?
-
?
monocaprin + H2O
-
Aspergillus nidulans glycerol + caproate
-
?
monoolein + H2O
-
Aspergillus nidulans glycerol + oleic acid
-
?
monoolein + H2O
-
Aspergillus nidulans PW1 glycerol + oleic acid
-
?
monopalmitoin + H2O
-
Aspergillus nidulans glycerol + palmitoleic acid
-
?
additional information the purified esterase is able to hydrolyze synthetic substrates with acyl group chain lengths of C4, C12, and C14 and displays higher activity (85% higher) toward C4 chain-lengths Aspergillus nidulans ?
-
?
additional information the purified esterase is able to hydrolyze synthetic substrates with acyl group chain lengths of C4, C12, and C14 and displays higher activity (85% higher) toward C4 chain-lengths Aspergillus nidulans PW1 ?
-
?
tributyrin + H2O
-
Aspergillus nidulans butanoate + glycerol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Aspergillus nidulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
Aspergillus nidulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 80 the enzyme retains 80% of its activity after incubation at 30°C to 70°C for 30 min and loses 50% of its activity after incubation for 15 min at 80°C, the enzyme is relatively stable below 60°C, while the inactivation rate increases dramatically above this temperature Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Aspergillus nidulans

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
Aspergillus nidulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 the enzyme retains activity after incubation at pHs ranging from 8 to 11 for 12 h at 37°C and 6 to 8 for 24 h at 37°C Aspergillus nidulans

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus nidulans isoelectric focusing
-
4.5