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Literature summary for 3.1.1.1 extracted from
- A versatile esterase from Bacillus subtilis: cloning, expression, characterization, and its application in biocatalysis (2007), Biotechnol. J., 2, 249-253.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | effective removal of t-butyl protecting group in organic synthesis | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G105A | 6fold increase in enantioselectivity against substrate (RS)-2-phenyl-3-butyn-2-yl acetate | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 119 | - |
p-nitrophenyl acetate | pH 7.4 | Bacillus subtilis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P37967 | isoform BS2, enzyme additionally shows amidase activity | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 2 weeks | Bacillus subtilis |
| -80°C, stable for at least 2 weeks | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phenyl-3-butyn-2-yl acetate | - |
Bacillus subtilis | 2-phenyl-3-butyn-2-ol + acetate | - |
? | |
| 3-methyl-1-pentyn-3-yl acetate + H2O | - |
Bacillus subtilis | 3-methyl-1-pentyn-3-ol + acetate | - |
? | |
| linalyl acetate + H2O | - |
Bacillus subtilis | linalool + acetate | - |
? | |
| additional information | enzyme activity against tertiary alcohol esters is about 1000fold lower compared with secondary and primary alcohol esters. The enantioselectivity towards esters of tertiary alcohols is very low | Bacillus subtilis | ? | - |
? | |
| p-nitrophenol butanoate + H2O | - |
Bacillus subtilis | p-nitrophenol + butanoate | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Bacillus subtilis | p-nitrophenol + acetate | - |
? | |
| t-butyl acetate + H2O | - |
Bacillus subtilis | t-butanol + acetate | - |
? | |
| t-butyl-gamma-(t-butoxycarbonylamino)-butyrate + H2O | - |
Bacillus subtilis | ? | effective removal of t-butyl protecting group in organic synthesis | ? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
active up to | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
40 | stable for at least 16 h | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
completely inactive above | Bacillus subtilis |
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