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Literature summary for 3.1.1.1 extracted from
- A novel esterase from Bacillus subtilis (RRL 1789): purification and characterization of the enzyme (2006), Protein Expr. Purif., 45, 262-268.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, complete inhibition | Bacillus subtilis |  |
| Fe3+ | partial | Bacillus subtilis | |
| Hg2+ | 1 mM, complete inhibition | Bacillus subtilis | |
| Mn2+ | partial | Bacillus subtilis | |
| additional information | not inhibitory: EDTA, EGTA, Li+, K+, NH4+, Mg2+, Triton X-100 at 0.1%, dimethylsulfoxide at 15% | Bacillus subtilis | |
| phenylmethylsulfonyl fluoride | 1 mM, complete inhibition | Bacillus subtilis | |
| sodium dodecylsulfate | 0.1% w/V, 10% residual activity | Bacillus subtilis | |
| Zn2+ | 1 mM, complete inhibition | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
4-nitrophenyl butanoate | 37°C | Bacillus subtilis | |
| 0.008 | - |
4-nitrophenyl caproate | 37°C | Bacillus subtilis | |
| 0.017 | - |
4-nitrophenyl caprylate | 37°C | Bacillus subtilis | |
| 0.05 | - |
4-nitrophenyl acetate | 37°C | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 20% stimulation | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
strasin RRL 1789 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 944 | - |
37°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Bacillus subtilis | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butanoate + H2O | - |
Bacillus subtilis | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl caproate + H2O | - |
Bacillus subtilis | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Bacillus subtilis | 4-nitrophenol + caprylate | - |
? | |
| triacetin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| tributyrin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| tricaproin + H2O | poor substrate | Bacillus subtilis | ? | - |
? | |
| tricaprylin + H2O | poor substrate | Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BSE | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
1 h, 50% residual activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Bacillus subtilis |
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Release 2023.1 (January 2023)
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