Literature summary for 3.1.1.1 extracted from

Pesaresi, A.; Devescovi, G.; Lamba, D.; Venturi, V.; Degrassi, G.
Isolation, characterization, and heterologous expression of a carboxylesterase of Pseudomonas aeruginosa PAO1 (2005), Curr. Microbiol., 50, 102-109.

Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas aeruginosa
expression in Escherichia coli	Pseudomonas aeruginosa PAO1

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	-	Pseudomonas aeruginosa PAO1
CoCl2	1mM, 34% residual activity	Pseudomonas aeruginosa
Cu2+	-	Pseudomonas aeruginosa PAO1
CuCl2	1 mM, 59% residual activity	Pseudomonas aeruginosa
dithiothreitol	1 mM, 58% residual activity	Pseudomonas aeruginosa
DTT	1 mM, 42% inhibition	Pseudomonas aeruginosa PAO1
Fe2+	-	Pseudomonas aeruginosa PAO1
Fe3+	-	Pseudomonas aeruginosa PAO1
FeCl2	1 mM, 40% residual activity	Pseudomonas aeruginosa
FeCl3	1 mM, 38% residual activity	Pseudomonas aeruginosa
additional information	not inhibitory: EDTA	Pseudomonas aeruginosa
additional information	no inhibition by EDTA	Pseudomonas aeruginosa PAO1
Ni2+	-	Pseudomonas aeruginosa PAO1
NiCl2	1 mM, 35% residual activity	Pseudomonas aeruginosa
phenylmethylsulfonyl fluoride	0.1 mM, 48% residual activity	Pseudomonas aeruginosa
phenylmethylsulfonyl fluoride	complete inhibition above 1 mM	Pseudomonas aeruginosa PAO1
Zn2+	-	Pseudomonas aeruginosa PAO1
ZnCl2	1 mM, 36% residual activity	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.43	-	p-nitrophenyl acetate	-	Pseudomonas aeruginosa PAO1
0.43	-	4-nitrophenyl acetate	pH 9.0, 25°C	Pseudomonas aeruginosa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
MgCl2	slight activation	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
19300	-	gel filtration	Pseudomonas aeruginosa
19300	-	gel filtration	Pseudomonas aeruginosa PAO1
24000	-	1 * 24000, SDS-PAGE	Pseudomonas aeruginosa PAO1
24691	-	1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS	Pseudomonas aeruginosa
28000	-	1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-
Pseudomonas aeruginosa PAO1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Pseudomonas aeruginosa PAO1
recombinant protein	Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7310	-	25°C, pH 7.0	Pseudomonas aeruginosa PAO1
7310	-	pH 9.0, 25°C	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-naphthyl acetate + H2O	-	Pseudomonas aeruginosa	1-naphthol + acetate	-	?
1-naphthyl butanoate + H2O	54% of the activity with 1-naphthyl acetate	Pseudomonas aeruginosa	1-naphthol + butanoate	-	?
1-naphthyl caprylate + H2O	14% of the activity with 1-naphthyl acetate	Pseudomonas aeruginosa	1-naphthol + caprylate	-	?
1-naphthyl laurate + H2O	9% of the activity with 1-naphthyl acetate	Pseudomonas aeruginosa	1-naphthol + laurate	-	?
1-naphthyl oleate + H2O	2% of the activity with 1-naphthyl acetate	Pseudomonas aeruginosa	1-naphthol + oleate	-	?
4-methylumbelliferyl acetate + H2O	-	Pseudomonas aeruginosa	4-methylumbelliferone + acetate	-	?
4-methylumbelliferyl acetate + H2O	-	Pseudomonas aeruginosa PAO1	4-methylumbelliferone + acetate	-	?
4-nitrophenyl acetate + H2O	-	Pseudomonas aeruginosa	4-nitrophenol + acetate	-	?
4-nitrophenyl butanoate + H2O	-	Pseudomonas aeruginosa	4-nitrophenol + butanoate	-	?
alpha-naphthyl acetate + H2O	-	Pseudomonas aeruginosa PAO1	alpha-naphthol + acetate	-	?
alpha-naphthyl butyrate + H2O	-	Pseudomonas aeruginosa PAO1	alpha-naphthol + butyrate	-	?
carboxyfluorescein diacetate + H2O	-	Pseudomonas aeruginosa	carboxyfluorescein + acetate	-	?
carboxyfluorescein diacetate + H2O	-	Pseudomonas aeruginosa PAO1	?	-	?
fluorescein diacetate + H2O	-	Pseudomonas aeruginosa PAO1	?	-	?
fluorescein diacetate + H2O	-	Pseudomonas aeruginosa	fluorescein + acetate	-	?
p-nitrophenyl acetate + H2O	-	Pseudomonas aeruginosa PAO1	p-nitrophenol + acetate	-	?
p-nitrophenyl butyrate	-	Pseudomonas aeruginosa PAO1	p-nitrophenol + butyrate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 24000, SDS-PAGE	Pseudomonas aeruginosa PAO1
monomer	1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Pseudomonas aeruginosa
55	-	-	Pseudomonas aeruginosa PAO1

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
45	65	45°C: about 60% of maximal activity, 65°C: about 50% of maximal activity	Pseudomonas aeruginosa PAO1

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	pH 9, 2 h, stable up to	Pseudomonas aeruginosa PAO1
50	-	pH 9.0, 2 h, stable up to 50°C	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
170	-	fluorescein diacetate	-	Pseudomonas aeruginosa
170	-	fluorescein diacetate	-	Pseudomonas aeruginosa PAO1
1600	-	1-naphthyl butanoate	-	Pseudomonas aeruginosa
1600	-	alpha-naphthyl butyrate	-	Pseudomonas aeruginosa PAO1
1700	-	p-nitrophenyl butyrate	-	Pseudomonas aeruginosa PAO1
1700	-	4-nitrophenyl butanoate	-	Pseudomonas aeruginosa
2800	-	carboxyfluorescein diacetate	-	Pseudomonas aeruginosa
2800	-	carboxyfluorescein diacetate	-	Pseudomonas aeruginosa PAO1
3000	-	Alpha-naphthyl acetate	-	Pseudomonas aeruginosa PAO1
3000	-	1-naphthyl acetate	-	Pseudomonas aeruginosa
6400	-	4-nitrophenyl acetate	-	Pseudomonas aeruginosa
6400	-	p-nitrophenyl acetate	-	Pseudomonas aeruginosa PAO1
8500	-	4-yethylumbelliferyl acetate	-	Pseudomonas aeruginosa
8500	-	4-yethylumbelliferyl acetate	-	Pseudomonas aeruginosa PAO1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Pseudomonas aeruginosa
9	-	-	Pseudomonas aeruginosa PAO1

pH Range

pH Minimum	pH Maximum	Comment	Organism
8	10	about 40% of maximal activity at pH 8.0 and 10.0	Pseudomonas aeruginosa PAO1

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	8	stable	Pseudomonas aeruginosa PAO1

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pseudomonas aeruginosa	calculated	-	5
Pseudomonas aeruginosa	isoelectric focusing	-	5.2
Pseudomonas aeruginosa PAO1	isoelectric focusing, pH-range 3-10	-	5.2

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Release 2023.1 (January 2023)