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Literature summary for 3.1.1.1 extracted from
- Intracellular esterase from Lactobacillus casei LILA: nucleotide sequencing, purification, and characterization (2003), J. Dairy Sci., 86, 1118-1129.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for NaCl | Lacticaseibacillus casei |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene estA, expression as His-tagged enzyme in Escherichia coli | Lactobacillus delbrueckii subsp. lactis |
| gene estC, DNA sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli | Lacticaseibacillus casei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphate | - |
Lactobacillus delbrueckii subsp. lactis |  |
| iodoacetic acid | slight inhibition | Lacticaseibacillus casei | |
| iodoacetic acid | - |
Lactobacillus delbrueckii subsp. lactis | |
| p-chloromercuribenzoate | - |
Lactobacillus delbrueckii subsp. lactis | |
| pepstatin A | - |
Lacticaseibacillus casei | |
| pepstatin A | - |
Lactobacillus delbrueckii subsp. lactis | |
| PMSF | - |
Lacticaseibacillus casei | |
| PMSF | - |
Lactobacillus delbrueckii subsp. lactis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
4-nitrophenyl dodecanoate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 0.032 | - |
4-nitrophenyl decanoate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 0.034 | - |
4-nitrophenyl hexanoate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 0.072 | - |
4-nitrophenyl octanoate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 0.077 | - |
4-nitrophenyl acetate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 0.079 | - |
4-nitrophenyl pentanoate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 0.097 | - |
4-nitrophenyl butanoate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 0.12 | - |
4-nitrophenyl hexanoate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 0.12 | - |
4-nitrophenyl propionate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 0.12 | - |
4-nitrophenyl pentanoate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 0.19 | - |
hexyl acetate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 0.28 | - |
4-nitrophenyl butanoate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 0.42 | - |
phenylthioacetate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 0.47 | - |
4-nitrophenyl acetate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 0.67 | - |
4-nitrophenyl propionate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 0.93 | - |
phenyl acetate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 2.8 | - |
ethyl hexanoate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 3.4 | - |
butyl acetate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 6 | - |
propyl acetate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 6.6 | - |
ethyl pentanoate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 6.8 | - |
ethyl butanoate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 7.5 | - |
ethyl hexanoate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 10 | - |
ethyl propionate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 13 | - |
phenylthioacetate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 19 | - |
ethyl pentanoate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 35 | - |
phenyl acetate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
| 39 | - |
ethyl acetate | pH 7.5, 30°C | Lacticaseibacillus casei | |
| 62 | - |
ethyl butanoate | pH 7.5, 30°C | Lactobacillus delbrueckii subsp. lactis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | optimal concentration at 30°C is 12.5% | Lactobacillus delbrueckii subsp. lactis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
2 * 29000, recombinant enzyme, SDS-PAGE | Lacticaseibacillus casei |
| 30000 | - |
2 * 30000, recombinant enzyme, SDS-PAGE | Lactobacillus delbrueckii subsp. lactis |
| 63000 | - |
recombinant enzyme, gel filtration | Lactobacillus delbrueckii subsp. lactis |
| 63000 | - |
recombinant enzyme, gel filtration | Lacticaseibacillus casei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | Q8KT44 | LILA, EstC | - |
| Lacticaseibacillus casei LILA EstC | Q8KT44 | LILA, EstC | - |
| Lactobacillus delbrueckii subsp. lactis | - |
MG1363, EstA | - |
| Lactobacillus delbrueckii subsp. lactis MG1363 EstA | - |
MG1363, EstA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme to homogeneity from Escherichia coli | Lactobacillus delbrueckii subsp. lactis |
| recombinant His-tagged enzyme to homogeneity from Escherichia coli | Lacticaseibacillus casei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37 | - |
purified recombinant enzyme | Lactobacillus delbrueckii subsp. lactis |
| 48.8 | - |
purified recombinant enzyme, in absence of NaCl | Lacticaseibacillus casei |
| 51 | - |
purified recombinant enzyme, 12.5% NaCl | Lactobacillus delbrueckii subsp. lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Lacticaseibacillus casei | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Lactobacillus delbrueckii subsp. lactis MG1363 EstA | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Lacticaseibacillus casei LILA EstC | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butanoate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl butanoate + H2O | best substrate | Lacticaseibacillus casei | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl decanoate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl dodecanoate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | 4-nitrophenol + dodecanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | - |
Lacticaseibacillus casei | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | best substrate | Lactobacillus delbrueckii subsp. lactis | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl pentanoate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | 4-nitrophenol + pentanoate | - |
? | |
| 4-nitrophenyl pentanoate + H2O | - |
Lacticaseibacillus casei | 4-nitrophenol + pentanoate | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | 4-nitrophenol + propionate | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Lacticaseibacillus casei | 4-nitrophenol + propionate | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Lactobacillus delbrueckii subsp. lactis MG1363 EstA | 4-nitrophenol + propionate | - |
? | |
| butyl acetate + H2O | - |
Lacticaseibacillus casei | butanol + acetate | - |
? | |
| ethyl acetate + H2O | - |
Lacticaseibacillus casei | ethanol + acetate | - |
? | |
| ethyl acetate + H2O | - |
Lacticaseibacillus casei LILA EstC | ethanol + acetate | - |
? | |
| ethyl butanoate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | ethanol + butanoate | - |
? | |
| ethyl butanoate + H2O | - |
Lacticaseibacillus casei | ethanol + butanoate | - |
? | |
| ethyl hexanoate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | ethanol + hexanoate | - |
? | |
| ethyl hexanoate + H2O | - |
Lacticaseibacillus casei | ethanol + hexanoate | - |
? | |
| ethyl pentanoate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | ethanol + pentanoate | - |
? | |
| ethyl pentanoate + H2O | - |
Lacticaseibacillus casei | ethanol + pentanoate | - |
? | |
| ethyl propionate + H2O | - |
Lacticaseibacillus casei | ethanol + propionate | - |
? | |
| hexyl acetate + H2O | - |
Lacticaseibacillus casei | hexanol + acetate | - |
? | |
| additional information | substrate specificity, poor activity with 4-nitrophenyl octanoate, 4-nitrophenyl decanoate, 4-nitrophenyl dodecanoate, and carboxylic esters with chain length above C14 | Lacticaseibacillus casei | ? | - |
? | |
| additional information | substrate specificity, poor substrates are ethyl acetate, ethylpropionate, carboxylic esters with chain length above C14, propyl acetate, butyl acetate, and hexanoyl acetate | Lactobacillus delbrueckii subsp. lactis | ? | - |
? | |
| additional information | substrate specificity, poor substrates are ethyl acetate, ethylpropionate, carboxylic esters with chain length above C14, propyl acetate, butyl acetate, and hexanoyl acetate | Lactobacillus delbrueckii subsp. lactis MG1363 EstA | ? | - |
? | |
| additional information | substrate specificity, poor activity with 4-nitrophenyl octanoate, 4-nitrophenyl decanoate, 4-nitrophenyl dodecanoate, and carboxylic esters with chain length above C14 | Lacticaseibacillus casei LILA EstC | ? | - |
? | |
| phenyl acetate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | phenol + acetate | - |
? | |
| phenyl acetate + H2O | - |
Lacticaseibacillus casei | phenol + acetate | - |
? | |
| phenyl acetate + H2O | - |
Lactobacillus delbrueckii subsp. lactis MG1363 EstA | phenol + acetate | - |
? | |
| phenyl acetate + H2O | - |
Lacticaseibacillus casei LILA EstC | phenol + acetate | - |
? | |
| phenylthioacetate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | ? | - |
? | |
| phenylthioacetate + H2O | - |
Lactobacillus delbrueckii subsp. lactis MG1363 EstA | ? | - |
? | |
| phenylthioacetate + H2O | - |
Lacticaseibacillus casei | thiophenol + acetate | - |
? | |
| phenylthioacetate + H2O | - |
Lacticaseibacillus casei LILA EstC | thiophenol + acetate | - |
? | |
| propyl acetate + H2O | - |
Lacticaseibacillus casei | propanol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 29000, recombinant enzyme, SDS-PAGE | Lacticaseibacillus casei |
| dimer | 2 * 30000, recombinant enzyme, SDS-PAGE | Lactobacillus delbrueckii subsp. lactis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EstA | - |
Lactobacillus delbrueckii subsp. lactis |
| EstC | - |
Lacticaseibacillus casei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
recombinant enzyme | Lacticaseibacillus casei |
| 45 | - |
- |
Lactobacillus delbrueckii subsp. lactis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 40 | - |
Lacticaseibacillus casei |
| 5 | 50 | - |
Lactobacillus delbrueckii subsp. lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
recombinant enzyme | Lacticaseibacillus casei |
| 7.5 | 8.5 | - |
Lactobacillus delbrueckii subsp. lactis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lactobacillus delbrueckii subsp. lactis | isoelectric focusing | - |
5.2 |
| Lacticaseibacillus casei | isoelectric focusing | - |
7.1 |
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