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Literature summary for 3.1.1.1 extracted from

  • Sousa, H.A.; Afonso, C.A.M.; Crespo, J.G.
    Kinetic study of the enantioselective hydrolysis of a meso-diester using pig liver Esterase (2000), J. Chem. Technol. Biotechnol., 75, 707-714.
No PubMed abstract available

Application

Application Comment Organism
synthesis methyl hydrogen (1S,2R)-cyclohex-4-ene-1,2-dicarboxylate is an important educt for the synthesis of diverse biologically relevant products such as carbacyclin, prostaglandin synthon, brefeldin, anticapsin and others Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
methanol noncompetitive product inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a carboxylic ester + H2O = an alcohol + a carboxylate reaction mechanism Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethyl cis-cyclohex-4-ene-1,2-dicarboxylate + H2O enantioselectivity, 98% at 25°C, 96% at 50°C Sus scrofa methyl hydrogen (1S,2R)-cyclohex-4-ene-1,2-dicarboxylate + methanol 94-97% of the R-enantiomer ir

Synonyms

Synonyms Comment Organism
pig liver esterase
-
Sus scrofa
PLE
-
Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50
-
Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
good long-term stability Sus scrofa
45 50 denaturation occurs Sus scrofa
55
-
rapid denaturation Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Sus scrofa