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Literature summary for 3.1.1.1 extracted from

  • Sugihara, A.; Shimada, Y.; Nagao, T.; Iizumi, T.; Nakamura, K.; Fukase, T.; Tominaga, Y.
    Purification and characterization of a carboxylesterase from Pseudomonas sp. KWI-56 (1994), Biosci. Biotechnol. Biochem., 58, 752-755.
    View publication on PubMed
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
overproduction in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
DFP
-
 Pseudomonas sp.
eserine
-
 Pseudomonas sp.
Hg2+ HgCl2 Pseudomonas sp.
PCMB
-
 Pseudomonas sp.
PMSF
-
 Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Pseudomonas sp.
50000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. KWI-56
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1110
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl propionate + H2O hydrolyzed at highest rate among the fatty acid methyl esters Pseudomonas sp. methanol + propionate
-
 ?
methyl propionate + H2O hydrolyzed at highest rate among the fatty acid methyl esters Pseudomonas sp. KWI-56 methanol + propionate
-
 ?
tributyrylglycerol + H2O
-
 Pseudomonas sp. butyrate + glycerol
-
 ?
tributyrylglycerol + H2O
-
 Pseudomonas sp. KWI-56 butyrate + glycerol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
36
-
 30 min, stable up to Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 20°C Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 pH 6.5: about 60% of maximal activity, pH 8.0: about 70% of maximal activity Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 25°C, 20 h, stable Pseudomonas sp.