Cloned (Comment) | Organism |
---|---|
overproduction in Escherichia coli | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DFP | - |
Pseudomonas sp. | |
eserine | - |
Pseudomonas sp. | |
Hg2+ | HgCl2 | Pseudomonas sp. | |
PCMB | - |
Pseudomonas sp. | |
PMSF | - |
Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Pseudomonas sp. |
50000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. KWI-56 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1110 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl propionate + H2O | hydrolyzed at highest rate among the fatty acid methyl esters | Pseudomonas sp. | methanol + propionate | - |
? | |
methyl propionate + H2O | hydrolyzed at highest rate among the fatty acid methyl esters | Pseudomonas sp. KWI-56 | methanol + propionate | - |
? | |
tributyrylglycerol + H2O | - |
Pseudomonas sp. | butyrate + glycerol | - |
? | |
tributyrylglycerol + H2O | - |
Pseudomonas sp. KWI-56 | butyrate + glycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
30 min, stable up to | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
20°C | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | pH 6.5: about 60% of maximal activity, pH 8.0: about 70% of maximal activity | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | 25°C, 20 h, stable | Pseudomonas sp. |