Inhibitors | Comment | Organism | Structure |
---|---|---|---|
eserine | - |
Geobacillus stearothermophilus | |
Hg2+ | HgCl2 | Geobacillus stearothermophilus | |
p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus | |
PMSF | - |
Geobacillus stearothermophilus | |
tosyl-L-Phe | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
4-nitrophenyl laurate | - |
Geobacillus stearothermophilus | |
0.011 | - |
4-nitrophenyl caprate | - |
Geobacillus stearothermophilus | |
0.012 | - |
4-nitrophenyl octanoate | - |
Geobacillus stearothermophilus | |
0.021 | - |
4-nitrophenyl hexanoate | - |
Geobacillus stearothermophilus | |
0.031 | - |
4-nitrophenyl pentanoate | - |
Geobacillus stearothermophilus | |
0.041 | - |
4-nitrophenyl butyrate | - |
Geobacillus stearothermophilus | |
0.05 | - |
4-nitrophenyl propionate | - |
Geobacillus stearothermophilus | |
0.055 | - |
4-nitrophenyl acetate | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Geobacillus stearothermophilus |
50000 | - |
1 * 50000, SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.032 | - |
- |
Geobacillus stearothermophilus |
1543 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + capric acid | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + lauric acid | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + propionate | - |
? | |
benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O | - |
Geobacillus stearothermophilus | benzyloxycarbonyl-Gly + 4-nitrophenol | - |
? | |
benzyloxycarbonyl-L-Ala-p-nitrophenyl ester + H2O | - |
Geobacillus stearothermophilus | benzyloxycarbonyl-L-Ala + 4-nitrophenol | - |
? | |
benzyloxycarbonyl-L-Tyr-p-nitrophenyl ester + H2O | - |
Geobacillus stearothermophilus | benzyloxycarbonyl-L-Tyr + 4-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
bovine serum albumin enhances thermostability | Geobacillus stearothermophilus |
56 | - |
pH 6.0, stable for 7 days | Geobacillus stearothermophilus |
60 | - |
pH 6.0, half-life: 170 h | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | influence of bovine serum albumin on kinetic parameters | Geobacillus stearothermophilus | |
555 | - |
4-nitrophenyl laurate | - |
Geobacillus stearothermophilus | |
750 | - |
4-nitrophenyl acetate | - |
Geobacillus stearothermophilus | |
800 | - |
4-nitrophenyl caprate | - |
Geobacillus stearothermophilus | |
1170 | - |
4-nitrophenyl propionate | - |
Geobacillus stearothermophilus | |
1250 | - |
4-nitrophenyl octanoate | - |
Geobacillus stearothermophilus | |
1380 | - |
4-nitrophenyl butyrate | - |
Geobacillus stearothermophilus | |
1550 | - |
4-nitrophenyl hexanoate | - |
Geobacillus stearothermophilus | |
15300 | - |
4-nitrophenyl pentanoate | - |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
optimum stability | Geobacillus stearothermophilus |