Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.9.1.3 extracted from

  • Jager, G.; Chen, P.; Bjork, G.R.
    Transfer RNA bound to mnmh protein is enriched with geranylated tRNA-a possible intermediate in its selenation (2016), PLoS One, 11, e0153488 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.9.1.3

Protein Variants

Protein Variants Comment Organism
G67E mutation in the rhodanese domain increases the intrinsic low geranylation activity of the wild type MnmH Salmonella enterica subsp. enterica serovar Typhimurium
G67E/K155A the mutant enzyme does not transfer in vitro a geranyl group from geranylpyrophosphate to tRNA Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
selenophosphate + geranyl diphosphate + 5-methylaminomethyl-2-thiouridine34 in tRNA + H2O Salmonella enterica subsp. enterica serovar Typhimurium the enzyme converts 5-methylaminomethyl-2-uridine and 5-carboxymethylaminomethyl-2-uridine to the respective selenouridine forms in a two-step process that involves geranylation and subsequent phosphoselenation of the resulting geranylated intermediates. The resultant seleno-phosphorylated uridine intermediates further react with a water molecule to release a phosphate anion and 2-selenouridine tRNA 5-methylaminomethyl-2-selenouridine34 in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol + diphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium Q8ZR88
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-methylaminomethyl-2-selenouridine34 in tRNA + phosphate
-
 ?
geranyl diphosphate + 5-methylaminomethyl-2-thiouridine34 in tRNA
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA + diphosphate
-
 ?
selenophosphate + 5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol
-
 ?
selenophosphate + geranyl diphosphate + 5-methylaminomethyl-2-thiouridine34 in tRNA + H2O the enzyme converts 5-methylaminomethyl-2-uridine and 5-carboxymethylaminomethyl-2-uridine to the respective selenouridine forms in a two-step process that involves geranylation and subsequent phosphoselenation of the resulting geranylated intermediates. The resultant seleno-phosphorylated uridine intermediates further react with a water molecule to release a phosphate anion and 2-selenouridine tRNA Salmonella enterica subsp. enterica serovar Typhimurium 5-methylaminomethyl-2-selenouridine34 in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol + diphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
selU
-
 Salmonella enterica subsp. enterica serovar Typhimurium
tRNA 2-selenouridine synthase
-
 Salmonella enterica subsp. enterica serovar Typhimurium