BRENDA - Enzyme Database show
show all sequences of 2.9.1.2

Leishmania donovani encodes a functional selenocysteinyl-tRNA synthase

Manhas, R.; Gowri, V.S.; Madhubala, R.; J. Biol. Chem. 291, 1203-1220 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli SelA-deficient strain JS1
Leishmania donovani
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Leishmania donovani
5737
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-phospho-L-seryl-tRNASec + selenophosphate + H2O
Leishmania donovani
-
L-selenocysteinyl-tRNASec + phosphate
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate + H2O
Leishmania donovani Bob
-
L-selenocysteinyl-tRNASec + phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leishmania donovani
-
-
-
Leishmania donovani Bob
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
promastigote
-
Leishmania donovani
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
O-phospho-L-seryl-tRNASec + selenophosphate + H2O
-
738683
Leishmania donovani
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate + H2O
-
738683
Leishmania donovani Bob
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
-
Leishmania donovani
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli SelA-deficient strain JS1
Leishmania donovani
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Leishmania donovani
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-phospho-L-seryl-tRNASec + selenophosphate + H2O
Leishmania donovani
-
L-selenocysteinyl-tRNASec + phosphate
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate + H2O
Leishmania donovani Bob
-
L-selenocysteinyl-tRNASec + phosphate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
promastigote
-
Leishmania donovani
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
O-phospho-L-seryl-tRNASec + selenophosphate + H2O
-
738683
Leishmania donovani
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate + H2O
-
738683
Leishmania donovani Bob
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
-
Leishmania donovani
General Information
General Information
Commentary
Organism
physiological function
the enzyme is dispensable for the parasite survival
Leishmania donovani
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is dispensable for the parasite survival
Leishmania donovani
Other publictions for EC 2.9.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738683
Manhas
Leishmania donovani encodes a ...
Leishmania donovani, Leishmania donovani Bob
J. Biol. Chem.
291
1203-1220
2016
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739263
Zhao
The role of Sep (O-phosphoseri ...
Homo sapiens
Placenta
34
967-972
2013
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723559
Li
Priority in selenium homeostas ...
Gallus gallus
PLoS ONE
7
e35761
2012
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723214
Hohn
Genetic analysis of selenocyst ...
Methanococcus maripaludis
Mol. Microbiol.
81
249-258
2011
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1
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694074
Aeby
The selenoproteome is dispensa ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
168
191-193
2009
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694917
Aeby
The canonical pathway for sele ...
Trypanosoma brucei
Proc. Natl. Acad. Sci. USA
106
5088-5092
2009
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695136
Palioura
The human SepSecS-tRNASec comp ...
Homo sapiens
Science
325
321-325
2009
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694428
Araiso
Structural insights into RNA-d ...
Methanococcus maripaludis
Nucleic Acids Res.
36
1187-1199
2008
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694847
Yuan
RNA-dependent conversion of ph ...
Homo sapiens, Methanocaldococcus jannaschii, Methanococcus maripaludis
Proc. Natl. Acad. Sci. USA
103
18923-18927
2006
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