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Genetic analysis of selenocysteine biosynthesis in the archaeon Methanococcus maripaludis

Hohn, M.J.; Palioura, S.; Su, D.; Yuan, J.; Soell, D.; Mol. Microbiol. 81, 249-258 (2011)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanococcus maripaludis
Q6LZM9
-
-
General Information
General Information
Commentary
Organism
physiological function
Methanococcus maripaludis Mm900 is facultatively selenium-dependent with a single pathway of Sec-tRNASec formation. Seelenocysteine formation is abolished upon individually deleting the genes encoding selenophosphate synthetase, phosphoseryl-tRNASec kinase, or SepSecS. The resulting mutant strains can no longer grow on formate while growth with H2 + CO2 remains unaffected. Deletion of the phosphoseryl-tRNASec kinase and SepSecS genes is not possible unless the selenium-free [NiFe]-hydrogenases Frc and Vhc are expressed
Methanococcus maripaludis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
Methanococcus maripaludis Mm900 is facultatively selenium-dependent with a single pathway of Sec-tRNASec formation. Seelenocysteine formation is abolished upon individually deleting the genes encoding selenophosphate synthetase, phosphoseryl-tRNASec kinase, or SepSecS. The resulting mutant strains can no longer grow on formate while growth with H2 + CO2 remains unaffected. Deletion of the phosphoseryl-tRNASec kinase and SepSecS genes is not possible unless the selenium-free [NiFe]-hydrogenases Frc and Vhc are expressed
Methanococcus maripaludis
Other publictions for EC 2.9.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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723214
Hohn
Genetic analysis of selenocyst ...
Methanococcus maripaludis
Mol. Microbiol.
81
249-258
2011
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694074
Aeby
The selenoproteome is dispensa ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
168
191-193
2009
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694917
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106
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2009
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695136
Palioura
The human SepSecS-tRNASec comp ...
Homo sapiens
Science
325
321-325
2009
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694428
Araiso
Structural insights into RNA-d ...
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36
1187-1199
2008
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694847
Yuan
RNA-dependent conversion of ph ...
Homo sapiens, Methanocaldococcus jannaschii, Methanococcus maripaludis
Proc. Natl. Acad. Sci. USA
103
18923-18927
2006
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