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Literature summary for 2.8.5.2 extracted from

  • Rother, D.; Friedrich, C.G.
    The cytochrome complex SoxXA of Paracoccus pantotrophus is produced in Escherichia coli and functional in the reconstituted sulfur-oxidizing enzyme system (2002), Biochim. Biophys. Acta, 1598, 65-73 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
the heterodimeric cytochrome complex SoxXA is produced in Escherichia coli. The soxX and soxA genes, separated by two genes in the sox gene cluster are fused with ribosome binding sites optimal for Escherichia coli and combined to give soxXA in pRD133.27. The cytochrome complex SoxXA is produced in Escherichia coli M15 containing pRD133.27, pREP4 encoding the Lac repressor and plasmid pEC86, carrying essential cytochrome c maturation genes. This cytochrome complex is equally catalytically active with respect to rates and reactivity with different sulfur substrates in the reconstituted sulfur-oxidizing enzyme system using homogeneous Sox-proteins of Paracoccus pantotrophus Paracoccus pantotrophus

General Stability

General Stability Organism
SoxA is unstable when not complexed with SoxX Paracoccus pantotrophus

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[SoxY protein]-L-cysteine + thiosulfate + 2 ferricytochrome c Paracoccus pantotrophus the enzyme is involved in the oxidation of reduced inorganic sulfur compounds [SoxY protein]-S-sulfosulfanyl-L-cysteine + 2 ferrocytochrome c + 2 H+
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 ?
[SoxY protein]-S-sulfanyl-L-cysteine + thiosulfate + 2 ferricytochrome c Paracoccus pantotrophus the enzyme is involved in the oxidation of reduced inorganic sulfur compounds [SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + 2 ferrocytochrome c + 2 H+
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 ?

Organism

Organism UniProt Comment Textmining
Paracoccus pantotrophus O33434 AND Q9LCV0 O33434: subunit SoxA, Q9LCV0: subunit SoxX
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Purification (Commentary)

Purification (Comment) Organism
the cytochrome complex SoxXA is purified to homogeneity from periplasmic extracts of Escherichia coli M15 Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[SoxY protein]-L-cysteine + thiosulfate + 2 ferricytochrome c
-
 Paracoccus pantotrophus [SoxY protein]-S-sulfosulfanyl-L-cysteine + 2 ferrocytochrome c + 2 H+
-
 ?
[SoxY protein]-L-cysteine + thiosulfate + 2 ferricytochrome c the enzyme is involved in the oxidation of reduced inorganic sulfur compounds Paracoccus pantotrophus [SoxY protein]-S-sulfosulfanyl-L-cysteine + 2 ferrocytochrome c + 2 H+
-
 ?
[SoxY protein]-S-sulfanyl-L-cysteine + thiosulfate + 2 ferricytochrome c
-
 Paracoccus pantotrophus [SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + 2 ferrocytochrome c + 2 H+
-
 ?
[SoxY protein]-S-sulfanyl-L-cysteine + thiosulfate + 2 ferricytochrome c the enzyme is involved in the oxidation of reduced inorganic sulfur compounds Paracoccus pantotrophus [SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + 2 ferrocytochrome c + 2 H+
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 29352 (subunit SoxA) + 1 * 14216 (subunit SoxX), calculated from sequence Paracoccus pantotrophus

Synonyms

Synonyms Comment Organism
complex SoxXA
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 Paracoccus pantotrophus

Cofactor

Cofactor Comment Organism Structure
heme one heme is assigned to subunit SoxX and two hemes to subunit SoxA Paracoccus pantotrophus

General Information

General Information Comment Organism
metabolism the enzyme is involved in the oxidation of reduced inorganic sulfur compounds Paracoccus pantotrophus