Literature summary for 2.8.4.1 extracted from

Rospert, S.; Voges, M.; Berkessel, A.; Albracht, S.P.J.; Thauer, R.K.
Substrate-analog-induced changes in the nickel-EPR spectrum of active methyl-coenzyme-M reductase from Methanobacterium thermoautotrophicum (1992), Eur. J. Biochem., 210, 101-107.

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General Stability

General Stability	Organism
methyl-coenzyme M stabilizes enzyme activity	Methanothermobacter thermautotrophicus

Inhibitors

Inhibitors	Comment	Organism	Structure
3-bromopropanesulfonate	complete inhibition at 0.02 mM; most potent inhibitor, competitive with respect to methyl-coenzyme M	Methanothermobacter thermautotrophicus
3-fluoropropanesulfonate	-	Methanothermobacter thermautotrophicus
3-iodopropanesulfonate	-	Methanothermobacter thermautotrophicus
7-bromoheptanoylthreonine phosphate	substrate analogue of coenzyme B in which the thiol group is substituted by bromine, potent inhibitor, competitive with respect to methyl-coenzyme M and B	Methanothermobacter thermautotrophicus
additional information	inhibitors are substrate analogues	Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4	-	methyl coenzyme M	-	Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ni(2+)	-	Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
300000	-	-	Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
methyl coenzyme M + coenzyme B	Methanothermobacter thermautotrophicus	final step in methane formation in all methanogenic archaea	methane + CoM-S-S-CoB	-	?
methyl coenzyme M + coenzyme B	Methanothermobacter thermautotrophicus Marburg / DSM 2133	final step in methane formation in all methanogenic archaea	methane + CoM-S-S-CoB	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanothermobacter thermautotrophicus	-	-	-
Methanothermobacter thermautotrophicus Marburg / DSM 2133	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
under 5% H2/95% N2 condition, fractionated ammonium sulfate precipitation	Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	the active state of the enzyme is the reduced state	Methanothermobacter thermautotrophicus
3	-	extract of H2-reduced cells	Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl coenzyme M + coenzyme B	final step in methane formation in all methanogenic archaea	Methanothermobacter thermautotrophicus	methane + CoM-S-S-CoB	-	?
methyl coenzyme M + coenzyme B	final step in methane formation in all methanogenic archaea	Methanothermobacter thermautotrophicus Marburg / DSM 2133	methane + CoM-S-S-CoB	-	?

Cofactor

Cofactor	Comment	Organism	Structure
F-430	-	Methanothermobacter thermautotrophicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00005	-	3-bromopropanesulfonate	-	Methanothermobacter thermautotrophicus
0.001	-	3-iodopropanesulfonate	below	Methanothermobacter thermautotrophicus
0.005	-	7-bromoheptanoylthreonine phosphate	-	Methanothermobacter thermautotrophicus
0.05	-	3-fluoropropanesulfonate	below	Methanothermobacter thermautotrophicus

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Release 2023.1 (January 2023)