Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.4.1 extracted from

  • Bonacker, L.G.; Baudner, S.; Morschel, E.; Bocher, R.; Thauer, R.K.
    Properties of the two isoenzymes of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum (1993), Eur. J. Biochem., 217, 587-595.
    View publication on PubMed
Search for more literature for 2.8.4.1

General Stability

General Stability Organism
activity is stabilized by high concentrations of ammonium sulfate Methanothermobacter thermautotrophicus

Inhibitors

Inhibitors Comment Organism Structure
3-bromopropanesulfonate most potent inhibitor, competitive with respect to methyl-coenzyme M Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1 0.3 coenzyme B isoenzyme I Methanothermobacter thermautotrophicus
0.4 0.6 coenzyme B isoenzyme II Methanothermobacter thermautotrophicus
0.6 0.8 methyl coenzyme M isoenzyme I Methanothermobacter thermautotrophicus
1.3 1.5 methyl coenzyme M isoenzyme II Methanothermobacter thermautotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Methanothermobacter thermautotrophicus 5737
-
membrane on the inner surface of the cytoplasmic membrane Methanothermobacter thermautotrophicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ni(2+) 2 mol Ni/mol enzyme Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
-
 Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus rate limiting step in methanogenesis methane + CoM-S-S-CoB
-
 ?
methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus energy metabolism of all methanogenic archaea methane + CoM-S-S-CoB
-
 ?
methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus Marburg / DSM 2133 rate limiting step in methanogenesis methane + CoM-S-S-CoB
-
 ?
methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus Marburg / DSM 2133 energy metabolism of all methanogenic archaea methane + CoM-S-S-CoB
-
 ?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus
-
 produces different isoenzymes under different growth conditions
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Oxidation Stability

Oxidation Stability Organism
high concentrations of both H2 and CO2 favour the expression of isoenzyme II, low concentrations favour the expression of isoenzyme I Methanothermobacter thermautotrophicus

Purification (Commentary)

Purification (Comment) Organism
copurification of isoenzymes, separation of isoenzymes by anion-exchange chromatography Methanothermobacter thermautotrophicus
hydrophobic interaction chromatography, Sepharose beads which contain antibodies specific for each isoenzyme, immunoadsorption method Methanothermobacter thermautotrophicus
under 5% H2/95% N2 condition, fractionated ammonium sulfate precipitation Methanothermobacter thermautotrophicus

Reaction

Reaction Comment Organism Reaction ID
methyl-CoM + CoB = CoM-S-S-CoB + methane ternary complex type Methanothermobacter thermautotrophicus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
 isoenzyme I Methanothermobacter thermautotrophicus
11.7
-
 isoenzyme II Methanothermobacter thermautotrophicus

Storage Stability

Storage Stability Organism
-70°C, rapidly frozen, yellow precipitate, suspended in 100%-saturated ammonium sulfate, stored unter N2, remains active Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl coenzyme M + coenzyme B rate limiting step in methanogenesis Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
methyl coenzyme M + coenzyme B energy metabolism of all methanogenic archaea Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
methyl coenzyme M + coenzyme B rate limiting step in methanogenesis Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?
methyl coenzyme M + coenzyme B energy metabolism of all methanogenic archaea Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?
methyl-coenzyme M + coenzyme B
-
 Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
methyl-coenzyme M + coenzyme B
-
 Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?

Subunits

Subunits Comment Organism
hexamer alpha2,beta2,gamma2 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Methanothermobacter thermautotrophicus
68 70 both isoenzymes Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 isoenzyme I Methanothermobacter thermautotrophicus
7
-
 assay at Methanothermobacter thermautotrophicus
7.5 8 isoenzyme II Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
F-430 nickel porphinoid coenzyme M Methanothermobacter thermautotrophicus
F-430 2 mol coenzyme F-430/mol enzyme Methanothermobacter thermautotrophicus
F-430 2 mol nickel porphinoid/mol enzyme, prosthetic group Methanothermobacter thermautotrophicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0001
-
 3-bromopropanesulfonate for both isoenzymes Methanothermobacter thermautotrophicus