Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ti(III) citrate | conversion from the ox1 to the red1 state, maximal activation after 30 min, 20 mM Ti(III) at pH 9.0 and room temperature or pH 7.0 and 60°C, optimal pH for activation of isoenzyme II is 10.0, enzyme is activated when the enzyme bound coenzyme F-430 is reduced to the Ni(I) state | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni(2+) | - |
Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
- |
Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus | methanogenesis | methane + CoM-S-S-CoB | - |
? | |
methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methanogenesis | methane + CoM-S-S-CoB | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
4°C, strictly anaerobic conditions, fractional ammonium-sulfate precipitation | Methanothermobacter thermautotrophicus |
copurification of isoenzymes, separation of isoenzymes by anion-exchange chromatography | Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity measured at 92% N2, 8% H2 | Methanothermobacter thermautotrophicus |
100 | - |
- |
Methanothermobacter thermautotrophicus |
Storage Stability | Organism |
---|---|
4°C, pH 7.0, 4 h, 50% loss of activity of red1 form | Methanothermobacter thermautotrophicus |
storage in frozen stage not possible, thawing leads to a rapid loss of activity | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl coenzyme M + coenzyme B | methanogenesis | Methanothermobacter thermautotrophicus | methane + CoM-S-S-CoB | - |
? | |
methyl coenzyme M + coenzyme B | methanogenesis | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methane + CoM-S-S-CoB | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha2,beta2,gamma2 | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Methanothermobacter thermautotrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH above 9.0 isoenzyme I is slowly denatured | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.2 | assay at | Methanothermobacter thermautotrophicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
above this pH value and 60°C isoenzyme I is slowly denatured | Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
F-430 | 2 mol nickel porphinoid/mol enzyme, prosthetic group | Methanothermobacter thermautotrophicus |