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Literature summary for 2.8.3.6 extracted from

  • Katagiri, M.; Hayaishi, O.
    Enzymatic degradation of beta-ketoadipic acid (1957), J. Biol. Chem., 226, 439-448.
    View publication on PubMed
Search for more literature for 2.8.3.6

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Pseudomonas fluorescens
-
-

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens 23
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
-10°C, at least 3 months Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates are acetoacetate, oxaloacetate or acetyl-CoA, and in the reverse reaction malonate, fumarate, oxalate or acetate Pseudomonas fluorescens ?
-
 ?
additional information no substrates are acetoacetate, oxaloacetate or acetyl-CoA, and in the reverse reaction malonate, fumarate, oxalate or acetate Pseudomonas fluorescens 23 ?
-
 ?
succinyl-CoA + 3-oxoadipate
-
 Pseudomonas fluorescens succinate + 3-oxoadipyl-CoA
-
 r
succinyl-CoA + 3-oxoadipate
-
 Pseudomonas fluorescens 23 succinate + 3-oxoadipyl-CoA
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas fluorescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, stable at neutral pH Pseudomonas fluorescens
80
-
 5 min, inactivation at neutral pH Pseudomonas fluorescens