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Literature summary for 2.8.3.5 extracted from
- New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols (2007), Protein Expr. Purif., 53, 396-403.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes scoA and scoB, encoding subunits of the heteromeric enzyme ScoAB, separate expression of the ScoAB subunits His-tagged ScoA and S-tagged ScoB | Bacillus subtilis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant ScoA and ScoB, sitting drop vapour diffusion method, 69 mg/ml protein in 20 mM Hepes, pH 8.0, 200 mM NaCl and 1 mM DTT, mixing with reservoir solution containing 100 mM Tris, pH 8.5, 200 mM MgCl2, and 20% PEG 8000, at 16°C, X-ray diffraction structure determination and analysis at 2.89 A resolution | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + acetoacetate | Bacillus subtilis | - |
succinate + acetoacetyl-CoA | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
genes scoA and scoB encode subunits of the heteromeric enzyme ScoAB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant ScoA and ScoB by metal affinity chromatography and gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + acetoacetate | - |
Bacillus subtilis | succinate + acetoacetyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | ScoAB subunits, separately expressed and mixed, do not interact effectively resulting in an inactive protein, overview | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ScoAB | - |
Bacillus subtilis |
| succinyl-CoA:acetoacetate coenzyme A transferase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.1 | - |
assay at | Bacillus subtilis |
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Release 2023.1 (January 2023)
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