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Literature summary for 2.8.3.5 extracted from
- Properties of succinyl-CoA:3-ketoacid coenzyme A transferase (1976), J. Biol. Chem., 251, 1708-1711.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
acetoacetyl-CoA | cosusbstrate: succinate | Sus scrofa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Sus scrofa |
| 45600 | - |
2 * 45600, sedimentation equilibrium in 6 M guanidine chloride | Sus scrofa |
| 78000 | - |
PAGE | Sus scrofa |
| 80000 | - |
gel filtration | Sus scrofa |
| 92000 | - |
sedimentation equilibrium centrifugation | Sus scrofa |
| 105000 | - |
SDS-PAGE after cross-linking with dimethyl dodecanediimidate | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains galactose, glucose, total sugar content: 1.6% | Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4 isozymes, separable by isoelectric focusing | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.9 | 4.5 | - |
Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + acetoacetate | - |
Sus scrofa | succinate + acetoacetyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45600, sedimentation equilibrium in 6 M guanidine chloride | Sus scrofa |
| dimer | 2 * 52000-63000, SDS-PAGE | Sus scrofa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sus scrofa | 4 isozymes with pI: 5.7, 5.9, 6.2 and 6.5 | - |
5.7 |
| Sus scrofa | 4 isozymes with pI: 5.7, 5.9, 6.2 and 6.5 | - |
5.9 |
| Sus scrofa | 4 isozymes with pI: 5.7, 5.9, 6.2 and 6.5 | - |
6.2 |
| Sus scrofa | 4 isozymes with pI: 5.7, 5.9, 6.2 and 6.5 | - |
6.5 |
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