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Literature summary for 2.8.3.19 extracted from

  • Mullins, E.A.; Sullivan, K.L.; Kappock, T.J.
    Function and X-ray crystal structure of Escherichia coli YfdE (2013), PLoS ONE, 8, e67901.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.3.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) or C41(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.5, 0.2 M MgCl2, and 20% (w/v) PEG 8000 Escherichia coli
hanging drop vapor diffusion method, using 0.1 M Tris-NHCl, pH 8.5, 0.2 M MgCl2, and 20% (w/v) PEG 8000 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 acetyl-CoA at pH 6.7 and 25°C Escherichia coli
22
-
 oxalate at pH 6.7 and 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetate + oxalyl-CoA Escherichia coli
-
 acetyl-CoA + oxalate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P76518
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Cibacron Blue 3GA column chromatography Escherichia coli
ammonium sulfate precipitation, Ni-NTA column chromatography, and Cibacron Blue 3GA column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.5
-
 at pH 6.7 and 25°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetate + oxalyl-CoA
-
 Escherichia coli acetyl-CoA + oxalate
-
 ?
additional information the enzyme shows low activity with formyl-CoA and acetate or oxalate, no activity with acetyl-CoA and succinate, formyl-CoA and succinate, or succinyl-CoA and oxalate Escherichia coli ?
-
 ?
oxalate + acetyl-CoA
-
 Escherichia coli oxalyl-CoA + acetate
-
 r

Subunits

Subunits Comment Organism
dimer x-ray crystallography Escherichia coli
homodimer x-ray crystallography Escherichia coli

Synonyms

Synonyms Comment Organism
acetyl-CoA:oxalate CoA transferase
-
 Escherichia coli
acetyl-CoA:oxalate CoA-transferase
-
 Escherichia coli
ACOCT
-
 Escherichia coli
YfdE
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
 acetyl-CoA at pH 6.7 and 25°C Escherichia coli
15
-
 oxalate at pH 6.7 and 25°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.68
-
 oxalate at pH 6.7 and 25°C Escherichia coli
650
-
 acetyl-CoA at pH 6.7 and 25°C Escherichia coli