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Literature summary for 2.8.3.16 extracted from

  • Mullins, E.A.; Starks, C.M.; Francois, J.A.; Sael, L.; Kihara, D.; Kappock, T.J.
    Formyl-coenzyme A (CoA):oxalate CoA-transferase from the acidophile Acetobacter aceti has a distinctive electrostatic surface and inherent acid stability (2012), Protein Sci., 21, 686-696.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acetobacter aceti

Crystallization (Commentary)

Crystallization (Comment) Organism
His6-tagged enzyme, in complex with CoA, hanging drop vapor diffusion method, using 24% (w/v) PEG 4000 and 100 mM bicine, pH 8.8 Acetobacter aceti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 formyl-CoA in 50 mM potassium phosphate, pH 6.7, at 25°C Acetobacter aceti
1.11
-
 oxalate in 50 mM potassium phosphate, pH 6.7, at 25°C Acetobacter aceti

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Acetobacter aceti 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, His6-tagged enzyme, SDS-PAGE Acetobacter aceti
49610
-
 2 * 49610, electrospray ionization mass spectrometry Acetobacter aceti
83000
-
 His6-tagged enzyme, gel filtration Acetobacter aceti
99000
-
 calculated from amino acid sequence Acetobacter aceti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formyl-CoA + oxalate Acetobacter aceti
-
 formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate Acetobacter aceti 1023
-
 formate + oxalyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Acetobacter aceti A9X6P7
-
-
Acetobacter aceti 1023 A9X6P7
-
-

Purification (Commentary)

Purification (Comment) Organism
iminodiacetate-Ni2+ Sepharose column chromatography Acetobacter aceti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formyl-CoA + oxalate
-
 Acetobacter aceti formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate
-
 Acetobacter aceti 1023 formate + oxalyl-CoA
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 47000, His6-tagged enzyme, SDS-PAGE Acetobacter aceti
homodimer 2 * 49610, electrospray ionization mass spectrometry Acetobacter aceti

Synonyms

Synonyms Comment Organism
FCOCT
-
 Acetobacter aceti
formyl-CoA:oxalate CoA-transferase
-
 Acetobacter aceti
uctB
-
 Acetobacter aceti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5
-
 formyl-CoA in 50 mM potassium phosphate, pH 6.7, at 25°C Acetobacter aceti
5
-
 oxalate in 50 mM potassium phosphate, pH 6.7, at 25°C Acetobacter aceti

Expression

Organism Comment Expression
Acetobacter aceti the enzyme is induced by acid stress up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.5
-
 oxalate in 50 mM potassium phosphate, pH 6.7, at 25°C Acetobacter aceti
1300
-
 formyl-CoA in 50 mM potassium phosphate, pH 6.7, at 25°C Acetobacter aceti