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Literature summary for 2.8.3.16 extracted from

  • Toyota, C.G.; Berthold, C.L.; Gruez, A.; Jonsson, S.; Lindqvist, Y.; Cambillau, C.; Richards, N.G.
    Differential substrate specificity and kinetic behavior of Escherichia coli YfdW and Oxalobacter formigenes formyl coenzyme A transferase (2008), J. Bacteriol., 190, 2556-2564.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expression Escherichia coli BL21-DE3 Oxalobacter formigenes
expression in Escherichia coli K12 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
FRC variants W48F and W48Q, hanging drop vapour diffusion method Oxalobacter formigenes

Protein Variants

Protein Variants Comment Organism
W48F FRC variant Oxalobacter formigenes
W48Q FRC variant Oxalobacter formigenes

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA
-
 Escherichia coli
coenzyme A free CoA Escherichia coli
oxalate substrate inhibition Escherichia coli
oxalate
-
 Oxalobacter formigenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 formyl-CoA wild-type FRC Oxalobacter formigenes
0.0047
-
 formyl-CoA His-FRC Oxalobacter formigenes
0.0058
-
 formyl-CoA W48Q FRC Oxalobacter formigenes
0.0171
-
 formyl-CoA W48F FRC Oxalobacter formigenes
0.35
-
 formyl-CoA His-YfdW Escherichia coli
0.43
-
 oxalate W48Q FRC Oxalobacter formigenes
0.51
-
 oxalate His-YfdW Escherichia coli
1.2
-
 oxalate His-FRC Oxalobacter formigenes
1.5
-
 oxalate W48F FRC Oxalobacter formigenes
3.9
-
 oxalate wild-type FRC Oxalobacter formigenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formyl-CoA + oxalate Oxalobacter formigenes FRC and oxalyl-CoA decarboxylase are essential for the survival of Oxalobacter in that they mediate the conversion of oxalate into formate and CO2 in a coupled catalytic cycle formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate Escherichia coli YfdW catalyzes the conversion of oxalate into oxalyl-CoA by using formyl-CoA as a donor, YfdW is a formyl-CoA transferase and YfdW appears to be more stringent than the corresponding enzyme (FRC) in Oxalobacter in employing formyl-CoA and oxalate as substrates formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate Escherichia coli MG1655 YfdW catalyzes the conversion of oxalate into oxalyl-CoA by using formyl-CoA as a donor, YfdW is a formyl-CoA transferase and YfdW appears to be more stringent than the corresponding enzyme (FRC) in Oxalobacter in employing formyl-CoA and oxalate as substrates formate + oxalyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 MG1655
-
Escherichia coli MG1655
-
 MG1655
-
Oxalobacter formigenes O06644
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged FRC protein is purified by Ni-nitrilotriacetic acid column and subsequent gel filtration Oxalobacter formigenes
His-tagged YfdW protein is purified by metal affinity chromatography and subsequent gel filtration on a Superdex 200 column, eluting with 5 mM HEPES buffer containing 150 mM NaCl, pH 7.5. Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formyl-CoA + oxalate
-
 Escherichia coli formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate
-
 Oxalobacter formigenes formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate FRC and oxalyl-CoA decarboxylase are essential for the survival of Oxalobacter in that they mediate the conversion of oxalate into formate and CO2 in a coupled catalytic cycle Oxalobacter formigenes formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate YfdW catalyzes the conversion of oxalate into oxalyl-CoA by using formyl-CoA as a donor, YfdW is a formyl-CoA transferase and YfdW appears to be more stringent than the corresponding enzyme (FRC) in Oxalobacter in employing formyl-CoA and oxalate as substrates Escherichia coli formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate
-
 Escherichia coli MG1655 formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate YfdW catalyzes the conversion of oxalate into oxalyl-CoA by using formyl-CoA as a donor, YfdW is a formyl-CoA transferase and YfdW appears to be more stringent than the corresponding enzyme (FRC) in Oxalobacter in employing formyl-CoA and oxalate as substrates Escherichia coli MG1655 formate + oxalyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
formyl coenzyme A transferase
-
 Escherichia coli
formyl-CoA-transferase
-
 Oxalobacter formigenes
FRC
-
 Oxalobacter formigenes
YfdW
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
23
-
 oxalate
-
 Escherichia coli
74
-
 oxalate W48Q FRC, the W48F FRC is not inhibited by oxalate at concentrations of up to 154 mM Oxalobacter formigenes