Cloned (Comment) | Organism |
---|---|
expression Escherichia coli BL21-DE3 | Oxalobacter formigenes |
expression in Escherichia coli K12 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
FRC variants W48F and W48Q, hanging drop vapour diffusion method | Oxalobacter formigenes |
Protein Variants | Comment | Organism |
---|---|---|
W48F | FRC variant | Oxalobacter formigenes |
W48Q | FRC variant | Oxalobacter formigenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Escherichia coli | |
coenzyme A | free CoA | Escherichia coli | |
oxalate | substrate inhibition | Escherichia coli | |
oxalate | - |
Oxalobacter formigenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
formyl-CoA | wild-type FRC | Oxalobacter formigenes | |
0.0047 | - |
formyl-CoA | His-FRC | Oxalobacter formigenes | |
0.0058 | - |
formyl-CoA | W48Q FRC | Oxalobacter formigenes | |
0.0171 | - |
formyl-CoA | W48F FRC | Oxalobacter formigenes | |
0.35 | - |
formyl-CoA | His-YfdW | Escherichia coli | |
0.43 | - |
oxalate | W48Q FRC | Oxalobacter formigenes | |
0.51 | - |
oxalate | His-YfdW | Escherichia coli | |
1.2 | - |
oxalate | His-FRC | Oxalobacter formigenes | |
1.5 | - |
oxalate | W48F FRC | Oxalobacter formigenes | |
3.9 | - |
oxalate | wild-type FRC | Oxalobacter formigenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-CoA + oxalate | Oxalobacter formigenes | FRC and oxalyl-CoA decarboxylase are essential for the survival of Oxalobacter in that they mediate the conversion of oxalate into formate and CO2 in a coupled catalytic cycle | formate + oxalyl-CoA | - |
? | |
formyl-CoA + oxalate | Escherichia coli | YfdW catalyzes the conversion of oxalate into oxalyl-CoA by using formyl-CoA as a donor, YfdW is a formyl-CoA transferase and YfdW appears to be more stringent than the corresponding enzyme (FRC) in Oxalobacter in employing formyl-CoA and oxalate as substrates | formate + oxalyl-CoA | - |
? | |
formyl-CoA + oxalate | Escherichia coli MG1655 | YfdW catalyzes the conversion of oxalate into oxalyl-CoA by using formyl-CoA as a donor, YfdW is a formyl-CoA transferase and YfdW appears to be more stringent than the corresponding enzyme (FRC) in Oxalobacter in employing formyl-CoA and oxalate as substrates | formate + oxalyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
MG1655 | - |
Escherichia coli MG1655 | - |
MG1655 | - |
Oxalobacter formigenes | O06644 | - |
- |
Purification (Comment) | Organism |
---|---|
His-tagged FRC protein is purified by Ni-nitrilotriacetic acid column and subsequent gel filtration | Oxalobacter formigenes |
His-tagged YfdW protein is purified by metal affinity chromatography and subsequent gel filtration on a Superdex 200 column, eluting with 5 mM HEPES buffer containing 150 mM NaCl, pH 7.5. | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-CoA + oxalate | - |
Escherichia coli | formate + oxalyl-CoA | - |
? | |
formyl-CoA + oxalate | - |
Oxalobacter formigenes | formate + oxalyl-CoA | - |
? | |
formyl-CoA + oxalate | FRC and oxalyl-CoA decarboxylase are essential for the survival of Oxalobacter in that they mediate the conversion of oxalate into formate and CO2 in a coupled catalytic cycle | Oxalobacter formigenes | formate + oxalyl-CoA | - |
? | |
formyl-CoA + oxalate | YfdW catalyzes the conversion of oxalate into oxalyl-CoA by using formyl-CoA as a donor, YfdW is a formyl-CoA transferase and YfdW appears to be more stringent than the corresponding enzyme (FRC) in Oxalobacter in employing formyl-CoA and oxalate as substrates | Escherichia coli | formate + oxalyl-CoA | - |
? | |
formyl-CoA + oxalate | - |
Escherichia coli MG1655 | formate + oxalyl-CoA | - |
? | |
formyl-CoA + oxalate | YfdW catalyzes the conversion of oxalate into oxalyl-CoA by using formyl-CoA as a donor, YfdW is a formyl-CoA transferase and YfdW appears to be more stringent than the corresponding enzyme (FRC) in Oxalobacter in employing formyl-CoA and oxalate as substrates | Escherichia coli MG1655 | formate + oxalyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
formyl coenzyme A transferase | - |
Escherichia coli |
formyl-CoA-transferase | - |
Oxalobacter formigenes |
FRC | - |
Oxalobacter formigenes |
YfdW | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
oxalate | - |
Escherichia coli | |
74 | - |
oxalate | W48Q FRC, the W48F FRC is not inhibited by oxalate at concentrations of up to 154 mM | Oxalobacter formigenes |