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Literature summary for 2.8.3.16 extracted from

  • Sidhu, H.; Ogden, S.D.; Lung, H.Y.; Luttge, B.G.; Baetz, A.L.; Peck, A.B.
    DNA sequencing and expression of the formyl coenzyme A transferase gene, frc, from, Oxalobacter formigenes (1997), J. Bacteriol., 179, 3378-3381.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.3.16

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, monocistronic operon, overexpression in Escherichia coli, activity is similar to the wild-type enzyme Oxalobacter formigenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 1 * 45000, recombinant enzyme, SDS-PAGE Oxalobacter formigenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formyl-CoA + oxalate Oxalobacter formigenes activation-decarboxylation reaction in the catabolism of oxalic acid, degradation and detoxification in mammalian intestinal flora formate + oxalyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Oxalobacter formigenes O06644 gene frc
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli JM109 Oxalobacter formigenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formyl-CoA + oxalate
-
 Oxalobacter formigenes formate + oxalyl-CoA
-
 ?
formyl-CoA + oxalate activation-decarboxylation reaction in the catabolism of oxalic acid, degradation and detoxification in mammalian intestinal flora Oxalobacter formigenes formate + oxalyl-CoA
-
 ?
succinyl-CoA + oxalate no activity with the recombinant enzyme expressed in Escherichia coli Oxalobacter formigenes succinate + oxalyl-CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, recombinant enzyme, SDS-PAGE Oxalobacter formigenes