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Literature summary for 2.8.3.15 extracted from

  • Leutwein, C.; Heider, J.
    Succinyl-CoA:(R)-benzylsuccinate CoA-transferase: an enzyme of the anaerobic toluene catabolic pathway in denitrifying bacteria (2001), J. Bacteriol., 183, 4288-4295.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.3.15

Inhibitors

Inhibitors Comment Organism Structure
(S)-2-benzylsuccinyl-CoA
-
 Thauera aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 (R)-2-benzylsuccinyl-CoA
-
 Thauera aromatica
0.16
-
 succinate
-
 Thauera aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 alpha2beta2, 2 * 44000 + 2 * 45000, SDS-PAGE, electrospray-mass spectroscopy Thauera aromatica
45000
-
 alpha2beta2, 2 * 44000 + 2 * 45000, SDS-PAGE, electrospray-mass spectroscopy Thauera aromatica
215000
-
 gel filtration Thauera aromatica

Organism

Organism UniProt Comment Textmining
Thauera aromatica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thauera aromatica

Reaction

Reaction Comment Organism Reaction ID
succinyl-CoA + (R)-2-benzylsuccinate = succinate + (R)-2-benzylsuccinyl-CoA mechanism Thauera aromatica
a
succinyl-CoA + (R)-2-benzylsuccinate = succinate + (R)-2-benzylsuccinyl-CoA mechanism, overview on CoA-transferase families Thauera aromatica
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
320
-
-
 Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maleyl-CoA + (R)-2-benzylsuccinate
-
 Thauera aromatica maleate + (R)-2-benzylsuccinyl-CoA
-
 r
methylsuccinyl-CoA + (R)-2-benzylsuccinate
-
 Thauera aromatica methylsuccinate + (R)-2-benzylsuccinyl-CoA
-
 r
succinyl-CoA + (R)-2-benzylsuccinate
-
 Thauera aromatica succinate + (R)-2-benzylsuccinyl-CoA
-
 r
succinyl-CoA + benzylmalonate
-
 Thauera aromatica succinate + benzylmalonyl-CoA
-
 r
succinyl-CoA + methylsuccinate
-
 Thauera aromatica succinate + methylsuccinyl-CoA
-
 r
succinyl-CoA + phenylsuccinate
-
 Thauera aromatica succinate + phenylsuccinyl-CoA
-
 r

Subunits

Subunits Comment Organism
heterotetramer alpha2beta2, 2 * 44000 + 2 * 45000, SDS-PAGE, electrospray-mass spectroscopy Thauera aromatica