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Literature summary for 2.8.3.12 extracted from

  • Mack, M.; Bendrat, K.; Zelder, O.; Eckel, E.; Linder, D.; Buckel, W.
    Location of the two genes encoding glutaconate coenzyme A-transferase at the beginning of the hydroxyglutarate operon in Acidaminococcus fermentans (1994), Eur. J. Biochem., 226, 41-51.
    View publication on PubMed
Search for more literature for 2.8.3.12

Cloned(Commentary)

Cloned (Comment) Organism
gctAB genes encoding the two subunits GctA and GctB are cloned, sequenced and overexpressed together in Escherichia coli DH5alpha, the two genes are located at the beginning of the hydroxyglutarate operon, GctA: 320 amino acids protein, GctB: 266 amino acids protein Acidaminococcus fermentans

Crystallization (Commentary)

Crystallization (Comment) Organism
native and recombinant enzyme, expressed in Escherichia coli Acidaminococcus fermentans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 alpha4beta4, 4 * 36000 + 4 * 29000, GctA and GctB, SDS-PAGE Acidaminococcus fermentans
29168
-
 alpha4beta4, 4 * 35725 + 4 * 29168, GctA and GctB, calculated from the DNA sequences Acidaminococcus fermentans
35725
-
 alpha4beta4, 4 * 35725 + 4 * 29168, GctA and GctB, calculated from the DNA sequences Acidaminococcus fermentans
36000
-
 alpha4beta4, 4 * 36000 + 4 * 29000, GctA and GctB, SDS-PAGE Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + (R)-2-hydroxyglutarate Acidaminococcus fermentans involved in the conversion of (R)-2-hydroxyglutarate to crotonyl-CoA in the pathway of glutamate fermentation acetate + (R)-2-hydroxyglutaryl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Acidaminococcus fermentans Q59111 and Q59112 5’-part of the hydroxyglutarate operon with gctAB genes encoding Gct, ATCC 25085
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant enzyme, expressed in Escherichia coli Acidaminococcus fermentans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Acidaminococcus fermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + (E)-glutaconate
-
 Acidaminococcus fermentans acetate + glutaconyl-1-CoA
-
 ?
acetyl-CoA + (R)-2-hydroxyglutarate
-
 Acidaminococcus fermentans acetate + (R)-2-hydroxyglutaryl-1-CoA
-
 ?
acetyl-CoA + (R)-2-hydroxyglutarate involved in the conversion of (R)-2-hydroxyglutarate to crotonyl-CoA in the pathway of glutamate fermentation Acidaminococcus fermentans acetate + (R)-2-hydroxyglutaryl-CoA
-
 ?
glutaryl-CoA + acetate
-
 Acidaminococcus fermentans glutarate + acetyl-CoA
-
 ?
additional information residue E-54 of the small subunit GctB is directly involved in catalysis Acidaminococcus fermentans ?
-
 ?

Subunits

Subunits Comment Organism
octamer alpha4beta4, 4 * 36000 + 4 * 29000, GctA and GctB, SDS-PAGE Acidaminococcus fermentans
octamer alpha4beta4, 4 * 35725 + 4 * 29168, GctA and GctB, calculated from the DNA sequences Acidaminococcus fermentans