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Literature summary for 2.8.2.B1 extracted from
- Adenosine 5-phosphosulfate sulfotransferase from the marine macroalga Porphyra yezoensis Ueda (Rhodophyta): stabilization, purification, and properties (1996), Planta, 198, 440-446.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| loss of activity upon freezing and thawing | Neopyropia yezoensis |
| the enzyme is irreversibly inactived by preincubation with thiols | Neopyropia yezoensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Neopyropia yezoensis |  |
| Cu2+ | - |
Neopyropia yezoensis | |
| Fe2+ | - |
Neopyropia yezoensis | |
| glutathione | slight substrate inhibition | Neopyropia yezoensis | |
| Hg2+ | - |
Neopyropia yezoensis | |
| Zn2+ | - |
Neopyropia yezoensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
adenosine 5'-phosphosulfate | pH 9.5, 25°C, with dithiothreitol as acceptor substrate | Neopyropia yezoensis | |
| 0.6 | - |
glutathione | - |
Neopyropia yezoensis | |
| 0.6 | - |
reduced L-glutathione | pH 9.5, 25°C | Neopyropia yezoensis | |
| 1.5 | - |
dithiothreitol | - |
Neopyropia yezoensis | |
| 1.5 | - |
dithioerythritol | - |
Neopyropia yezoensis | |
| 1.5 | - |
dithiothreitol | pH 9.5, 25°C | Neopyropia yezoensis | |
| 1.5 | - |
dithioerythritol | pH 9.5, 25°C | Neopyropia yezoensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | does not require bivalent cations | Neopyropia yezoensis | |
| additional information | no requirement for divalent cations. Mg2+, Ca2+, Ba2+ and Mn2+ have no effect | Neopyropia yezoensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Neopyropia yezoensis |
| 43000 | - |
8 * 43000, SDS-PAGE | Neopyropia yezoensis |
| 350000 | - |
gel filtration | Neopyropia yezoensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neopyropia yezoensis | - |
- |
- |
| Neopyropia yezoensis | - |
Ueda | - |
| Neopyropia yezoensis Ueda | - |
Ueda | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neopyropia yezoensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thallus | - |
Neopyropia yezoensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.3 | - |
- |
Neopyropia yezoensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50% of the activity is lost within 10 d, when the purified APSSTase is kept under N2 in 0.2M KH2PO4/K/HPO4 buffer (pH 8.0) containing 1.0 M (NH4)2SO4. The half-life for activity loss is slightly extended by the storage in an ice bath (0°C) | Neopyropia yezoensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine 5'-phosphosulfate + dithioerythritol | the enzyme is specific for adenosine 5'-phosphosulfate | Neopyropia yezoensis | ? | - |
? | |
| adenosine 5'-phosphosulfate + dithioerythritol | the enzyme is specific for adenosine 5'-phosphosulfate | Neopyropia yezoensis Ueda | ? | - |
? | |
| adenosine 5'-phosphosulfate + dithioerythritol | no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate | Neopyropia yezoensis | AMP + ? | - |
? | |
| adenosine 5'-phosphosulfate + dithioerythritol | no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate | Neopyropia yezoensis Ueda | AMP + ? | - |
? | |
| adenosine 5'-phosphosulfate + dithiothreitol | the enzyme is specific for adenosine 5'-phosphosulfate | Neopyropia yezoensis | ? | - |
? | |
| adenosine 5'-phosphosulfate + dithiothreitol | the enzyme is specific for adenosine 5'-phosphosulfate | Neopyropia yezoensis Ueda | ? | - |
? | |
| adenosine 5'-phosphosulfate + dithiothreitol | no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate | Neopyropia yezoensis | AMP + ? | - |
? | |
| adenosine 5'-phosphosulfate + dithiothreitol | no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate | Neopyropia yezoensis Ueda | AMP + ? | - |
? | |
| adenosine 5'-phosphosulfate + L-glutathione | the enzyme is specific for adenosine 5'-phosphosulfate | Neopyropia yezoensis | ? | - |
? | |
| adenosine 5'-phosphosulfate + L-glutathione | the enzyme is specific for adenosine 5'-phosphosulfate | Neopyropia yezoensis Ueda | ? | - |
? | |
| adenosine 5'-phosphosulfate + reduced L-glutathione | no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate | Neopyropia yezoensis | AMP + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 43000, SDS-PAGE | Neopyropia yezoensis |
| oligomer | x * 43000, SDS-PAGE | Neopyropia yezoensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APSSTase | - |
Neopyropia yezoensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal inactivation is depressed to a significant extent by a high concentration of Na2SO4. The effect is maximal in the concentration range of 0.4 M-0.6 M. Ammonium sulfate is also effective as an inhibitor of the thermal inactivation. Thermal inactivation is also depressed to some extent by adenosine 5'-phosphosulfate or its analogs, beta-methylene-adenosine 5'-phosphosulfate and AMP. The acceptor substrate dithiothreitol accelerates the thermal inactivation | Neopyropia yezoensis |
| additional information | - |
thermal inactivation is depressed to a significant extent by a high concentration of Na2SO4. The effect is maximal in the concentration range of 0.4-0.6 M. Ammonium sulfate is effective as an inhibitor of the thermal inactivation, but NaC1, CH3COONa or NaNO3 is not. The thermal inactivation is also depressed to some extent by adenosine 5'-phosphosulfate or AMP. The effect is concentration-dependent up to at least 2.0 mM | Neopyropia yezoensis |
| 25 | - |
pH 8.0, half-life: 8 min | Neopyropia yezoensis |
| 25 | - |
pH 9.0, about 75% loss of activity after 5 min | Neopyropia yezoensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.8 | - |
Neopyropia yezoensis |
| 9.5 | - |
- |
Neopyropia yezoensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | pH 7.0: about 20% of maximal activity, pH 8.0: 52% of maximal activity, pH 9.5: optimum | Neopyropia yezoensis |
| 7 | 9.8 | pH 7.0: about 20% of maximal activity, pH 8.0: 52% of maximal activity, pH 9.0-9.8: optimum | Neopyropia yezoensis |
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