Literature summary for 2.8.2.9 extracted from

Sekura, R.D.; Duffel, M.W.; Jakoby, W.B.
Aryl sulfotransferases (1981), Methods Enzymol., 77, 197-206.

Search for more literature for 2.8.2.9

Inhibitors

Inhibitors	Comment	Organism	Structure
2,6-Dichloro-4-nitrophenol	-	Rattus norvegicus
adenosine 3',5'-bisphosphate	-	Rattus norvegicus
Aryl sulfates	-	Rattus norvegicus
Pentachlorophenol	-	Rattus norvegicus
thiol reagents	-	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.024	-	3'-phosphoadenylylsulfate	pH 5.5, 37°C	Rattus norvegicus
0.1	-	2-naphthol	pH 5.5, 37°C	Rattus norvegicus
0.34	-	4-Chlorophenol	-	Rattus norvegicus
0.43	-	epinephrine	pH 5.5, 37°C	Rattus norvegicus
0.46	-	tyramine	pH 5.5, 37°C	Rattus norvegicus
0.91	-	tyrosine methyl ester	-	Rattus norvegicus
0.92	-	phenol	pH 5.5, 37°C	Rattus norvegicus
1.4	-	4-methoxyphenol	pH 5.5, 37°C	Rattus norvegicus
6.9	-	2-cyanoethyl-N-hydroxythioacetamide	pH 5.5, 37°C	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33500	-	2 * 33500, SDS-PAGE	Rattus norvegicus
61000	-	gel filtration	Rattus norvegicus

Organic Solvent Stability

Organic Solvent	Comment	Organism
Acetone	5% acetone is not inhibitory	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
aryl sulfotransferase IV	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.54	-	-	Rattus norvegicus

Storage Stability

Storage Stability	Organism
4°C, sodium phosphate buffer, pH 7.0, 0.25 M sucrose, 5 mM 2-mercaptoethanol, 3 mM NaN3, 5% loss of activity per week at protein concentration between 0.5 and 1 mg/ml	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylylsulfate + 2-cyanoethyl-N-hydroxythioacetamide	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylylsulfate + 2-cyanoethyl-N-hydroxythioacetimidate	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylylsulfate + 2-naphthol	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + 2-naphthyl sulfate	-	?
3'-phosphoadenylylsulfate + 4-chlorophenol	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate	-	?
3'-phosphoadenylylsulfate + 4-methoxyphenol	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + 4-methoxyphenyl sulfate	-	?
3'-phosphoadenylylsulfate + epinephrine	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylylsulfate + L-tyrosine methyl ester	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + L-tyrosine methyl ester 4-sulfate	-	?
3'-phosphoadenylylsulfate + phenol	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + phenyl sulfate	-	?
3'-phosphoadenylylsulfate + tyramine	-	Rattus norvegicus	adenosine 3',5'-bisphosphate + ?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 33500, SDS-PAGE	Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.03	-	phenol	-	Rattus norvegicus
0.0467	-	tyramine	pH 5.5, 37°C	Rattus norvegicus
0.122	-	epinephrine	pH 5.5, 37°C	Rattus norvegicus
0.233	-	tyrosine methyl ester	pH 5.5, 37°C	Rattus norvegicus
0.233	-	2-cyanoethyl-N-hydroxythioacetimidate	pH 5.5, 37°C	Rattus norvegicus
0.3	-	4-Chlorophenol	-	Rattus norvegicus
0.45	-	4-methoxyphenol	pH 5.5, 37°C	Rattus norvegicus
0.7	-	2-naphthol	pH 5.5, 37°C	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	2-naphthol, no formation of sulfate esters from tyrosine methyl ester, epinephrine, octopamine, tyramine, serotonin, N-acetylserotonin	Rattus norvegicus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rattus norvegicus	-	-	5.8

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Release 2023.1 (January 2023)