Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | activates below 0.1 M, inhibits at higher concentrations | Rattus norvegicus | |
NaCl | activates below 0.1 M, inhibits at higher concentrations | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2-naphthol | pH 5.5, 37°C | Rattus norvegicus | |
0.16 | - |
dopamine | pH 5.5, 37°C | Rattus norvegicus | |
0.17 | - |
4-nitrophenol | pH 5.5, 37°C | Rattus norvegicus | |
0.2 | - |
3-methoxy-4-hydroxyphenylglycol | pH 5.5, 37°C | Rattus norvegicus | |
0.21 | - |
5-hydroxytryptophol | pH 5.5, 37°C | Rattus norvegicus | |
0.26 | - |
2-Chloro-4-nitrophenyl sulfate | pH 7, 25°C | Rattus norvegicus | |
0.34 | - |
4-Chlorophenol | pH 5.5, 37°C | Rattus norvegicus | |
0.39 | 0.4 | 3-chlorophenol | pH 5.5, 37°C | Rattus norvegicus | |
0.43 | - |
epinephrine | pH 5.5, 37°C | Rattus norvegicus | |
0.44 | - |
3-Nitrophenol | pH 5.5, 37°C | Rattus norvegicus | |
0.46 | - |
tyramine | pH 5.5, 37°C | Rattus norvegicus | |
0.91 | - |
tyrosine methyl ester | pH 5.5, 37°C | Rattus norvegicus | |
0.92 | - |
phenol | pH 5.5, 37°C | Rattus norvegicus | |
1.2 | - |
4-Methylphenol | pH 5.5, 37°C | Rattus norvegicus | |
1.4 | - |
4-methoxyphenol | pH 5.5, 37°C | Rattus norvegicus | |
1.5 | - |
N-acetylserotonin | pH 5.5, 37°C | Rattus norvegicus | |
6.9 | - |
2-cyanoethyl-N-hydroxythioacetamide | pH 5.5, 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activates below 0.1 M, inhibits at higher concentrations | Rattus norvegicus | |
NaCl | activates below 0.1 M, inhibits at higher concentrations | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
2 * 33500, SDS-PAGE | Rattus norvegicus |
61000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.55 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + 2-chloro-4-nitrophenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 2-chloro-4-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 2-cyanoethyl-N-hydroxythioacetamide | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + 2-naphthol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 2-naphthyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 3-chlorophenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 3-chlorophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 3-methoxy-4-hydroxyphenylglycol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + 3-methylphenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 3-methylphenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 3-nitrophenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 4-chlorophenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 4-methoxyphenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 4-methoxyphenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 4-methylphenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 4-methylphenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 4-nitrophenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + 5-hydroxytryptophol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + dopamine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + epinephrine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + L-tyrosine methyl ester | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + L-tyrosine methyl ester 4-sulfate | - |
? | |
3'-phosphoadenylylsulfate + methylphenol | 3-methylphenol or 4-methylphenol | Rattus norvegicus | adenosine 3',5'-bisphosphate + methylphenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + N-acetylserotonin | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + nitrophenol | 3-nitrophenol or 4-nitrophenol | Rattus norvegicus | adenosine 3',5'-bisphosphate + nitrophenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + phenol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + phenyl sulfate | - |
? | |
3'-phosphoadenylylsulfate + tyramine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
additional information | catalyzes sulfate ester formation with simple phenols at an acidic pH and with several physiological metabolites at a more alkaline pH | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33500, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
4-Methylphenol | pH 5.5, 37°C | Rattus norvegicus | |
0.0233 | - |
2-cyanoethyl-N-hydroxythioacetamide | pH 5.5, 37°C | Rattus norvegicus | |
0.03 | - |
phenol | pH 5.5, 37°C | Rattus norvegicus | |
0.0467 | - |
tyramine | pH 5.5, 37°C | Rattus norvegicus | |
0.07 | - |
dopamine | pH 5.5, 37°C | Rattus norvegicus | |
0.122 | - |
epinephrine | pH 5.5, 37°C | Rattus norvegicus | |
0.14 | - |
5-hydroxytryptophol | pH 5.5, 37°C | Rattus norvegicus | |
0.183 | - |
3-methylphenol | pH 5.5, 37°C | Rattus norvegicus | |
0.183 | - |
3-methoxy-4-hydroxyphenolglycol | pH 5.5, 37°C | Rattus norvegicus | |
0.233 | - |
tyrosine methyl ester | pH 5.5, 37°C | Rattus norvegicus | |
0.3 | - |
N-acetylserotonin | pH 5.5, 37°C | Rattus norvegicus | |
0.317 | - |
4-Chlorophenol | pH 5.5, 37°C | Rattus norvegicus | |
0.45 | - |
3-chlorophenol | pH 5.5, 37°C | Rattus norvegicus | |
0.45 | - |
4-methoxyphenol | pH 5.5, 37°C | Rattus norvegicus | |
0.517 | - |
4-nitrophenol | pH 5.5, 37°C | Rattus norvegicus | |
0.7 | - |
2-naphthol | pH 5.5, 37°C | Rattus norvegicus | |
0.8 | - |
3-Nitrophenol | pH 5.5, 37°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
2-naphthol, sodium acetate buffer | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | pH gradient from pH 3.5 to pH 10 in a linear sucrose gradient from 5% to 40% | - |
5.8 |