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Literature summary for 2.8.2.9 extracted from

  • Sekura, R.D.; Jakoby, W.B.
    Aryl sulfotransferase IV from rat liver (1981), Arch. Biochem. Biophys., 211, 352-359.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
KCl activates below 0.1 M, inhibits at higher concentrations Rattus norvegicus
NaCl activates below 0.1 M, inhibits at higher concentrations Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
2-naphthol pH 5.5, 37°C Rattus norvegicus
0.16
-
dopamine pH 5.5, 37°C Rattus norvegicus
0.17
-
4-nitrophenol pH 5.5, 37°C Rattus norvegicus
0.2
-
3-methoxy-4-hydroxyphenylglycol pH 5.5, 37°C Rattus norvegicus
0.21
-
5-hydroxytryptophol pH 5.5, 37°C Rattus norvegicus
0.26
-
2-Chloro-4-nitrophenyl sulfate pH 7, 25°C Rattus norvegicus
0.34
-
4-Chlorophenol pH 5.5, 37°C Rattus norvegicus
0.39 0.4 3-chlorophenol pH 5.5, 37°C Rattus norvegicus
0.43
-
epinephrine pH 5.5, 37°C Rattus norvegicus
0.44
-
3-Nitrophenol pH 5.5, 37°C Rattus norvegicus
0.46
-
tyramine pH 5.5, 37°C Rattus norvegicus
0.91
-
tyrosine methyl ester pH 5.5, 37°C Rattus norvegicus
0.92
-
phenol pH 5.5, 37°C Rattus norvegicus
1.2
-
4-Methylphenol pH 5.5, 37°C Rattus norvegicus
1.4
-
4-methoxyphenol pH 5.5, 37°C Rattus norvegicus
1.5
-
N-acetylserotonin pH 5.5, 37°C Rattus norvegicus
6.9
-
2-cyanoethyl-N-hydroxythioacetamide pH 5.5, 37°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activates below 0.1 M, inhibits at higher concentrations Rattus norvegicus
NaCl activates below 0.1 M, inhibits at higher concentrations Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
2 * 33500, SDS-PAGE Rattus norvegicus
61000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 2-chloro-4-nitrophenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 2-chloro-4-nitrophenyl sulfate
-
?
3'-phosphoadenylylsulfate + 2-cyanoethyl-N-hydroxythioacetamide
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + 2-naphthol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
?
3'-phosphoadenylylsulfate + 3-chlorophenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 3-chlorophenyl sulfate
-
?
3'-phosphoadenylylsulfate + 3-methoxy-4-hydroxyphenylglycol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + 3-methylphenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 3-methylphenyl sulfate
-
?
3'-phosphoadenylylsulfate + 3-nitrophenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate
-
?
3'-phosphoadenylylsulfate + 4-chlorophenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
-
?
3'-phosphoadenylylsulfate + 4-methoxyphenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 4-methoxyphenyl sulfate
-
?
3'-phosphoadenylylsulfate + 4-methylphenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 4-methylphenyl sulfate
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
?
3'-phosphoadenylylsulfate + 5-hydroxytryptophol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + dopamine
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + epinephrine
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + L-tyrosine methyl ester
-
Rattus norvegicus adenosine 3',5'-bisphosphate + L-tyrosine methyl ester 4-sulfate
-
?
3'-phosphoadenylylsulfate + methylphenol 3-methylphenol or 4-methylphenol Rattus norvegicus adenosine 3',5'-bisphosphate + methylphenyl sulfate
-
?
3'-phosphoadenylylsulfate + N-acetylserotonin
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
3'-phosphoadenylylsulfate + nitrophenol 3-nitrophenol or 4-nitrophenol Rattus norvegicus adenosine 3',5'-bisphosphate + nitrophenyl sulfate
-
?
3'-phosphoadenylylsulfate + phenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + phenyl sulfate
-
?
3'-phosphoadenylylsulfate + tyramine
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
additional information catalyzes sulfate ester formation with simple phenols at an acidic pH and with several physiological metabolites at a more alkaline pH Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 33500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
4-Methylphenol pH 5.5, 37°C Rattus norvegicus
0.0233
-
2-cyanoethyl-N-hydroxythioacetamide pH 5.5, 37°C Rattus norvegicus
0.03
-
phenol pH 5.5, 37°C Rattus norvegicus
0.0467
-
tyramine pH 5.5, 37°C Rattus norvegicus
0.07
-
dopamine pH 5.5, 37°C Rattus norvegicus
0.122
-
epinephrine pH 5.5, 37°C Rattus norvegicus
0.14
-
5-hydroxytryptophol pH 5.5, 37°C Rattus norvegicus
0.183
-
3-methylphenol pH 5.5, 37°C Rattus norvegicus
0.183
-
3-methoxy-4-hydroxyphenolglycol pH 5.5, 37°C Rattus norvegicus
0.233
-
tyrosine methyl ester pH 5.5, 37°C Rattus norvegicus
0.3
-
N-acetylserotonin pH 5.5, 37°C Rattus norvegicus
0.317
-
4-Chlorophenol pH 5.5, 37°C Rattus norvegicus
0.45
-
3-chlorophenol pH 5.5, 37°C Rattus norvegicus
0.45
-
4-methoxyphenol pH 5.5, 37°C Rattus norvegicus
0.517
-
4-nitrophenol pH 5.5, 37°C Rattus norvegicus
0.7
-
2-naphthol pH 5.5, 37°C Rattus norvegicus
0.8
-
3-Nitrophenol pH 5.5, 37°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
2-naphthol, sodium acetate buffer Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus pH gradient from pH 3.5 to pH 10 in a linear sucrose gradient from 5% to 40%
-
5.8