General Stability | Organism |
---|---|
age-inactivated enzyme can be restored to full activity by 10 min preincubation with 50 mM mercaptoethanol, prolonged incubation for 24 h results in irreversible denaturation | Penicillium chrysogenum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-AMP | weak inhibitor | Penicillium chrysogenum | |
3',5'-ADP | - |
Penicillium chrysogenum | |
3'-AMP | - |
Penicillium chrysogenum | |
5'-ADP | - |
Penicillium chrysogenum | |
5'-AMP | - |
Penicillium chrysogenum | |
Chlorocholine | competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS | Penicillium chrysogenum | |
choline | substrate inhibitor | Penicillium chrysogenum | |
choline-O-phosphate | dead-end inhibitor, competitive inhibition with choline, linear mixed type inhibitor with respect to PAPS | Penicillium chrysogenum | |
additional information | 3,3-dimethylbutanol does not inhibit, enzyme is not significantly inhibited by methyl, butyl, pentyl or hexyl sulfates nor by p-nitrophenol or carnitine, EDTA is not inhibitory, sodium chloride, bromide and iodide have no effect | Penicillium chrysogenum | |
N,N-diethylethanolamine | - |
Penicillium chrysogenum | |
p-chloromercuribenzoate | - |
Penicillium chrysogenum | |
Tetramethylammonium | competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS | Penicillium chrysogenum | |
thiocholine | linear mixed type inhibitor with respect to PAPS | Penicillium chrysogenum | |
Trimethylammonium | competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS | Penicillium chrysogenum | |
vinyl trimethylammonium | neurine, competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS | Penicillium chrysogenum | |
vinyl trimethylammonium bromide | - |
Penicillium chrysogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
3'-phosphoadenylylsulfate | pH 7.8, 37°C | Penicillium chrysogenum | |
0.013 | - |
3'-phosphoadenylylsulfate | pH 7.8, 37°C, N,N-dimethylaminoethanol as substrate | Penicillium chrysogenum | |
0.017 | - |
choline | pH 7.8, 37°C | Penicillium chrysogenum | |
0.8 | - |
N,N-Dimethylaminoethanol | pH 7.8, 37°C | Penicillium chrysogenum | |
2.8 | - |
N,N,N-triethylaminoethanol | pH 7.8, 37°C | Penicillium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Penicillium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + choline | Penicillium chrysogenum | - |
adenosine 3',5'-bisphosphate + choline sulfate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Penicillium chrysogenum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Penicillium chrysogenum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000825 | - |
- |
Penicillium chrysogenum |
Storage Stability | Organism |
---|---|
-20°C, 25% sucrose, loses only about 20% of its activity after 1 month | Penicillium chrysogenum |
0°C, purified enzyme relatively stable when stored in presence of 25% sucrose | Penicillium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + choline | - |
Penicillium chrysogenum | adenosine 3',5'-bisphosphate + choline sulfate | - |
ir | |
3'-phosphoadenylylsulfate + choline | equilibrium of the choline sulfokinase reaction lies far toward the direction of choline-O-sulfate formation, only 0.1% conversion of choline-O-sulfate to 3'-phosphoadenylylsulfate can be detected | Penicillium chrysogenum | adenosine 3',5'-bisphosphate + choline sulfate | - |
ir | |
3'-phosphoadenylylsulfate + N,N,N-triethylaminoethanol | 2% of the activity with choline | Penicillium chrysogenum | adenosine 3',5'-bisphosphate + N,N,N-triethylaminoethanol O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol | 35% of the activity with choline | Penicillium chrysogenum | adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAPS:choline sulfotransferase | - |
Penicillium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | 7.2 | - |
Penicillium chrysogenum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | - |
Penicillium chrysogenum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8.8 | stable for at least 15 min | Penicillium chrysogenum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
choline-O-phosphate | pH 7.8, 37°C | Penicillium chrysogenum | |
2.5 | 14 | 3',5'-ADP | pH 7.8, 37°C depending on the choline concentration | Penicillium chrysogenum | |
4.1 | - |
3'-AMP | pH 7.8, 37°C | Penicillium chrysogenum | |
5.8 | - |
thiocholine | pH 7.8, 37°C | Penicillium chrysogenum | |
9.4 | - |
choline-O-phosphate | pH 7.8, 37°C | Penicillium chrysogenum |