Reference to 2.8.2.6; Id = 643829 - BRENDA Enzyme Database

age-inactivated enzyme can be restored to full activity by 10 min preincubation with 50 mM mercaptoethanol, prolonged incubation for 24 h results in irreversible denaturation

Penicillium chrysogenum

2'-AMP

weak inhibitor

Penicillium chrysogenum

3',5'-ADP

-

Penicillium chrysogenum

3'-AMP

-

Penicillium chrysogenum

5'-ADP

-

Penicillium chrysogenum

5'-AMP

-

Penicillium chrysogenum

Chlorocholine

competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS

Penicillium chrysogenum

choline

substrate inhibitor

Penicillium chrysogenum

choline-O-phosphate

dead-end inhibitor, competitive inhibition with choline, linear mixed type inhibitor with respect to PAPS

Penicillium chrysogenum

additional information

3,3-dimethylbutanol does not inhibit, enzyme is not significantly inhibited by methyl, butyl, pentyl or hexyl sulfates nor by p-nitrophenol or carnitine, EDTA is not inhibitory, sodium chloride, bromide and iodide have no effect

Penicillium chrysogenum

N,N-diethylethanolamine

-

Penicillium chrysogenum

p-chloromercuribenzoate

-

Penicillium chrysogenum

Tetramethylammonium

competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS

Penicillium chrysogenum

thiocholine

linear mixed type inhibitor with respect to PAPS

Penicillium chrysogenum

Trimethylammonium

competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS

Penicillium chrysogenum

vinyl trimethylammonium

neurine, competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS

Penicillium chrysogenum

vinyl trimethylammonium bromide

-

Penicillium chrysogenum

0.012

-

3'-phosphoadenylylsulfate

pH 7.8, 37Â°C

Penicillium chrysogenum

0.013

-

3'-phosphoadenylylsulfate

pH 7.8, 37Â°C, N,N-dimethylaminoethanol as substrate

Penicillium chrysogenum

0.017

-

choline

pH 7.8, 37Â°C

Penicillium chrysogenum

0.8

-

N,N-Dimethylaminoethanol

pH 7.8, 37Â°C

Penicillium chrysogenum

2.8

-

N,N,N-triethylaminoethanol

pH 7.8, 37Â°C

Penicillium chrysogenum

90000

-

gel filtration

Penicillium chrysogenum

3'-phosphoadenylylsulfate + choline

Penicillium chrysogenum

-

adenosine 3',5'-bisphosphate + choline sulfate

-

Penicillium chrysogenum

ir

Penicillium chrysogenum

-

partial

Penicillium chrysogenum

mycelium

-

Penicillium chrysogenum

-

0.000825

-

Penicillium chrysogenum

-20Â°C, 25% sucrose, loses only about 20% of its activity after 1 month

Penicillium chrysogenum

0Â°C, purified enzyme relatively stable when stored in presence of 25% sucrose

Penicillium chrysogenum

3'-phosphoadenylylsulfate + choline

-

643829

Penicillium chrysogenum

adenosine 3',5'-bisphosphate + choline sulfate

-

643829

Penicillium chrysogenum

ir

3'-phosphoadenylylsulfate + choline

equilibrium of the choline sulfokinase reaction lies far toward the direction of choline-O-sulfate formation, only 0.1% conversion of choline-O-sulfate to 3'-phosphoadenylylsulfate can be detected

643829

Penicillium chrysogenum

adenosine 3',5'-bisphosphate + choline sulfate

-

643829

Penicillium chrysogenum

ir

3'-phosphoadenylylsulfate + N,N,N-triethylaminoethanol

2% of the activity with choline

643829

Penicillium chrysogenum

adenosine 3',5'-bisphosphate + N,N,N-triethylaminoethanol O-sulfate

-

?

3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol

35% of the activity with choline

643829

Penicillium chrysogenum

adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate

-

?

PAPS:choline sulfotransferase

-

Penicillium chrysogenum

7.1

7.2

-

Penicillium chrysogenum

6

9.5

-

Penicillium chrysogenum

7

8.8

stable for at least 15 min

Penicillium chrysogenum

0.42

-

choline-O-phosphate

pH 7.8, 37Â°C

Penicillium chrysogenum

2.5

14

3',5'-ADP

pH 7.8, 37Â°C depending on the choline concentration

Penicillium chrysogenum

4.1

-

3'-AMP

pH 7.8, 37Â°C

Penicillium chrysogenum

5.8

-

thiocholine

pH 7.8, 37Â°C

Penicillium chrysogenum

9.4

-

choline-O-phosphate

pH 7.8, 37Â°C

Penicillium chrysogenum

age-inactivated enzyme can be restored to full activity by 10 min preincubation with 50 mM mercaptoethanol, prolonged incubation for 24 h results in irreversible denaturation

Penicillium chrysogenum

2'-AMP

weak inhibitor

Penicillium chrysogenum

3',5'-ADP

-

Penicillium chrysogenum

3'-AMP

-

Penicillium chrysogenum

5'-ADP

-

Penicillium chrysogenum

5'-AMP

-

Penicillium chrysogenum

Chlorocholine

competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS

Penicillium chrysogenum

choline

substrate inhibitor

Penicillium chrysogenum

choline-O-phosphate

dead-end inhibitor, competitive inhibition with choline, linear mixed type inhibitor with respect to PAPS

Penicillium chrysogenum

additional information

3,3-dimethylbutanol does not inhibit, enzyme is not significantly inhibited by methyl, butyl, pentyl or hexyl sulfates nor by p-nitrophenol or carnitine, EDTA is not inhibitory, sodium chloride, bromide and iodide have no effect

Penicillium chrysogenum

N,N-diethylethanolamine

-

Penicillium chrysogenum

p-chloromercuribenzoate

-

Penicillium chrysogenum

Tetramethylammonium

competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS

Penicillium chrysogenum

thiocholine

linear mixed type inhibitor with respect to PAPS

Penicillium chrysogenum

Trimethylammonium

competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS

Penicillium chrysogenum

vinyl trimethylammonium

neurine, competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS

Penicillium chrysogenum

vinyl trimethylammonium bromide

-

Penicillium chrysogenum

0.42

-

choline-O-phosphate

pH 7.8, 37Â°C

Penicillium chrysogenum

2.5

14

3',5'-ADP

pH 7.8, 37Â°C depending on the choline concentration

Penicillium chrysogenum

4.1

-

3'-AMP

pH 7.8, 37Â°C

Penicillium chrysogenum

5.8

-

thiocholine

pH 7.8, 37Â°C

Penicillium chrysogenum

9.4

-

choline-O-phosphate

pH 7.8, 37Â°C

Penicillium chrysogenum

0.012

-

3'-phosphoadenylylsulfate

pH 7.8, 37Â°C

Penicillium chrysogenum

0.013

-

3'-phosphoadenylylsulfate

pH 7.8, 37Â°C, N,N-dimethylaminoethanol as substrate

Penicillium chrysogenum

0.017

-

choline

pH 7.8, 37Â°C

Penicillium chrysogenum

0.8

-

N,N-Dimethylaminoethanol

pH 7.8, 37Â°C

Penicillium chrysogenum

2.8

-

N,N,N-triethylaminoethanol

pH 7.8, 37Â°C

Penicillium chrysogenum

90000

-

gel filtration

Penicillium chrysogenum

3'-phosphoadenylylsulfate + choline

Penicillium chrysogenum

-

adenosine 3',5'-bisphosphate + choline sulfate

-

Penicillium chrysogenum

ir

partial

Penicillium chrysogenum

mycelium

-

Penicillium chrysogenum

-

0.000825

-

Penicillium chrysogenum

-20Â°C, 25% sucrose, loses only about 20% of its activity after 1 month

Penicillium chrysogenum

0Â°C, purified enzyme relatively stable when stored in presence of 25% sucrose

Penicillium chrysogenum

3'-phosphoadenylylsulfate + choline

-

643829

Penicillium chrysogenum

adenosine 3',5'-bisphosphate + choline sulfate

-

643829

Penicillium chrysogenum

ir

3'-phosphoadenylylsulfate + choline

equilibrium of the choline sulfokinase reaction lies far toward the direction of choline-O-sulfate formation, only 0.1% conversion of choline-O-sulfate to 3'-phosphoadenylylsulfate can be detected

643829

Penicillium chrysogenum

adenosine 3',5'-bisphosphate + choline sulfate

-

643829

Penicillium chrysogenum

ir

3'-phosphoadenylylsulfate + N,N,N-triethylaminoethanol

2% of the activity with choline

643829

Penicillium chrysogenum

adenosine 3',5'-bisphosphate + N,N,N-triethylaminoethanol O-sulfate

-

?

3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol

35% of the activity with choline

643829

Penicillium chrysogenum

adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate

-

?

7.1

7.2

-

Penicillium chrysogenum

6

9.5

-

Penicillium chrysogenum

7

8.8

stable for at least 15 min

Penicillium chrysogenum

643830

Rivoal

Choline-O-sulfate biosynthesis ...

Limonium perezii, Limonium latifolium, Limonium nashii, Limonium ramosissimum, Limonium sinuatum, no activity in Brassica napus, no activity in Brassica oleracea, no activity in Helianthus annuus, no activity in Hordeum vulgare, no activity in Zea mays

Plant Physiol.

106

1187-1193

1994

1

-

5

2

-

1

-

1

-

20

-

15

-

2

-

1

-

1

-

5

-

2

-

1

-

1

-

15

-

2

-

1

-

643828

Fitzgerald

Further studies on the formati ...

Pseudomonas sp., Pseudomonas sp. C12B

Can. J. Microbiol.

23

483-490

1977

2

-

1

4

-

1

-

2

-

3

-

2

4

-

1

-

2

-

1

-

4

-

1

-

2

-

2

4

-

1

-

643829

Renosto

Choline sulfokinase of Penicil ...

Penicillium chrysogenum

Arch. Biochem. Biophys.

180

416-428

1977

-

1

16

5

-

1

-

1

-

1

-

1

2

4

-

1

-

1

-

5

-

1

-

16

5

-

1

-

1

-

1

2

4

-

1

-

643827

Orsi

Choline sulphokinase (sulfotra ...

Aspergillus nidulans

J. Biochem.

56

81-91

1964

3

-

31

4

-

1

-

1

-

1

-

1

-

3

4

-

1

-

3

-

1

-

3

-

31

-

4

-

1

-

1

-

1

-

3

4

-

1

-

3

-

1

-

Choline sulfokinase of Penicillium chrysogenum: partial purification and kinetic mechanism

Data extracted from this reference: