BRENDA - Enzyme Database
show all sequences of 2.8.2.6

Choline sulfokinase of Penicillium chrysogenum: partial purification and kinetic mechanism

Renosto, F.; Segel, I.H.; Arch. Biochem. Biophys. 180, 416-428 (1977) View publication on PubMed

Data extracted from this reference:

General Stability
General Stability
Organism
age-inactivated enzyme can be restored to full activity by 10 min preincubation with 50 mM mercaptoethanol, prolonged incubation for 24 h results in irreversible denaturation
Penicillium chrysogenum
Inhibitors
Inhibitors
Commentary
Organism
Structure
2'-AMP
weak inhibitor
Penicillium chrysogenum
3',5'-ADP
-
Penicillium chrysogenum
3'-AMP
-
Penicillium chrysogenum
5'-ADP
-
Penicillium chrysogenum
5'-AMP
-
Penicillium chrysogenum
Chlorocholine
competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
Penicillium chrysogenum
choline
substrate inhibitor
Penicillium chrysogenum
choline-O-phosphate
dead-end inhibitor, competitive inhibition with choline, linear mixed type inhibitor with respect to PAPS
Penicillium chrysogenum
additional information
3,3-dimethylbutanol does not inhibit, enzyme is not significantly inhibited by methyl, butyl, pentyl or hexyl sulfates nor by p-nitrophenol or carnitine, EDTA is not inhibitory, sodium chloride, bromide and iodide have no effect
Penicillium chrysogenum
N,N-diethylethanolamine
-
Penicillium chrysogenum
p-chloromercuribenzoate
-
Penicillium chrysogenum
Tetramethylammonium
competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
Penicillium chrysogenum
thiocholine
linear mixed type inhibitor with respect to PAPS
Penicillium chrysogenum
Trimethylammonium
competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
Penicillium chrysogenum
vinyl trimethylammonium
neurine, competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
Penicillium chrysogenum
vinyl trimethylammonium bromide
-
Penicillium chrysogenum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
3'-phosphoadenylylsulfate
pH 7.8, 37°C
Penicillium chrysogenum
0.013
-
3'-phosphoadenylylsulfate
pH 7.8, 37°C, N,N-dimethylaminoethanol as substrate
Penicillium chrysogenum
0.017
-
choline
pH 7.8, 37°C
Penicillium chrysogenum
0.8
-
N,N-Dimethylaminoethanol
pH 7.8, 37°C
Penicillium chrysogenum
2.8
-
N,N,N-triethylaminoethanol
pH 7.8, 37°C
Penicillium chrysogenum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
gel filtration
Penicillium chrysogenum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3'-phosphoadenylylsulfate + choline
Penicillium chrysogenum
-
adenosine 3',5'-bisphosphate + choline sulfate
-
Penicillium chrysogenum
ir
Organism
Organism
UniProt
Commentary
Textmining
Penicillium chrysogenum
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Penicillium chrysogenum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Penicillium chrysogenum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000825
-
-
Penicillium chrysogenum
Storage Stability
Storage Stability
Organism
-20°C, 25% sucrose, loses only about 20% of its activity after 1 month
Penicillium chrysogenum
0°C, purified enzyme relatively stable when stored in presence of 25% sucrose
Penicillium chrysogenum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3'-phosphoadenylylsulfate + choline
-
643829
Penicillium chrysogenum
adenosine 3',5'-bisphosphate + choline sulfate
-
643829
Penicillium chrysogenum
ir
3'-phosphoadenylylsulfate + choline
equilibrium of the choline sulfokinase reaction lies far toward the direction of choline-O-sulfate formation, only 0.1% conversion of choline-O-sulfate to 3'-phosphoadenylylsulfate can be detected
643829
Penicillium chrysogenum
adenosine 3',5'-bisphosphate + choline sulfate
-
643829
Penicillium chrysogenum
ir
3'-phosphoadenylylsulfate + N,N,N-triethylaminoethanol
2% of the activity with choline
643829
Penicillium chrysogenum
adenosine 3',5'-bisphosphate + N,N,N-triethylaminoethanol O-sulfate
-
-
-
?
3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol
35% of the activity with choline
643829
Penicillium chrysogenum
adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
PAPS:choline sulfotransferase
-
Penicillium chrysogenum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
7.2
-
Penicillium chrysogenum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
-
Penicillium chrysogenum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
8.8
stable for at least 15 min
Penicillium chrysogenum
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.42
-
choline-O-phosphate
pH 7.8, 37°C
Penicillium chrysogenum
2.5
14
3',5'-ADP
pH 7.8, 37°C depending on the choline concentration
Penicillium chrysogenum
4.1
-
3'-AMP
pH 7.8, 37°C
Penicillium chrysogenum
5.8
-
thiocholine
pH 7.8, 37°C
Penicillium chrysogenum
9.4
-
choline-O-phosphate
pH 7.8, 37°C
Penicillium chrysogenum
General Stability (protein specific)
General Stability
Organism
age-inactivated enzyme can be restored to full activity by 10 min preincubation with 50 mM mercaptoethanol, prolonged incubation for 24 h results in irreversible denaturation
Penicillium chrysogenum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2'-AMP
weak inhibitor
Penicillium chrysogenum
3',5'-ADP
-
Penicillium chrysogenum
3'-AMP
-
Penicillium chrysogenum
5'-ADP
-
Penicillium chrysogenum
5'-AMP
-
Penicillium chrysogenum
Chlorocholine
competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
Penicillium chrysogenum
choline
substrate inhibitor
Penicillium chrysogenum
choline-O-phosphate
dead-end inhibitor, competitive inhibition with choline, linear mixed type inhibitor with respect to PAPS
Penicillium chrysogenum
additional information
3,3-dimethylbutanol does not inhibit, enzyme is not significantly inhibited by methyl, butyl, pentyl or hexyl sulfates nor by p-nitrophenol or carnitine, EDTA is not inhibitory, sodium chloride, bromide and iodide have no effect
Penicillium chrysogenum
N,N-diethylethanolamine
-
Penicillium chrysogenum
p-chloromercuribenzoate
-
Penicillium chrysogenum
Tetramethylammonium
competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
Penicillium chrysogenum
thiocholine
linear mixed type inhibitor with respect to PAPS
Penicillium chrysogenum
Trimethylammonium
competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
Penicillium chrysogenum
vinyl trimethylammonium
neurine, competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
Penicillium chrysogenum
vinyl trimethylammonium bromide
-
Penicillium chrysogenum
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.42
-
choline-O-phosphate
pH 7.8, 37°C
Penicillium chrysogenum
2.5
14
3',5'-ADP
pH 7.8, 37°C depending on the choline concentration
Penicillium chrysogenum
4.1
-
3'-AMP
pH 7.8, 37°C
Penicillium chrysogenum
5.8
-
thiocholine
pH 7.8, 37°C
Penicillium chrysogenum
9.4
-
choline-O-phosphate
pH 7.8, 37°C
Penicillium chrysogenum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
3'-phosphoadenylylsulfate
pH 7.8, 37°C
Penicillium chrysogenum
0.013
-
3'-phosphoadenylylsulfate
pH 7.8, 37°C, N,N-dimethylaminoethanol as substrate
Penicillium chrysogenum
0.017
-
choline
pH 7.8, 37°C
Penicillium chrysogenum
0.8
-
N,N-Dimethylaminoethanol
pH 7.8, 37°C
Penicillium chrysogenum
2.8
-
N,N,N-triethylaminoethanol
pH 7.8, 37°C
Penicillium chrysogenum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
gel filtration
Penicillium chrysogenum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3'-phosphoadenylylsulfate + choline
Penicillium chrysogenum
-
adenosine 3',5'-bisphosphate + choline sulfate
-
Penicillium chrysogenum
ir
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Penicillium chrysogenum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Penicillium chrysogenum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000825
-
-
Penicillium chrysogenum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 25% sucrose, loses only about 20% of its activity after 1 month
Penicillium chrysogenum
0°C, purified enzyme relatively stable when stored in presence of 25% sucrose
Penicillium chrysogenum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3'-phosphoadenylylsulfate + choline
-
643829
Penicillium chrysogenum
adenosine 3',5'-bisphosphate + choline sulfate
-
643829
Penicillium chrysogenum
ir
3'-phosphoadenylylsulfate + choline
equilibrium of the choline sulfokinase reaction lies far toward the direction of choline-O-sulfate formation, only 0.1% conversion of choline-O-sulfate to 3'-phosphoadenylylsulfate can be detected
643829
Penicillium chrysogenum
adenosine 3',5'-bisphosphate + choline sulfate
-
643829
Penicillium chrysogenum
ir
3'-phosphoadenylylsulfate + N,N,N-triethylaminoethanol
2% of the activity with choline
643829
Penicillium chrysogenum
adenosine 3',5'-bisphosphate + N,N,N-triethylaminoethanol O-sulfate
-
-
-
?
3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol
35% of the activity with choline
643829
Penicillium chrysogenum
adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
7.2
-
Penicillium chrysogenum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
-
Penicillium chrysogenum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
8.8
stable for at least 15 min
Penicillium chrysogenum
Other publictions for EC 2.8.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
643830
Rivoal
Choline-O-sulfate biosynthesis ...
Limonium perezii, Limonium latifolium, Limonium nashii, Limonium ramosissimum, Limonium sinuatum, no activity in Brassica napus, no activity in Brassica oleracea, no activity in Helianthus annuus, no activity in Hordeum vulgare, no activity in Zea mays
Plant Physiol.
106
1187-1193
1994
1
-
-
-
-
-
5
2
-
1
-
1
-
20
-
-
-
-
-
15
-
-
2
-
-
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
-
-
5
-
2
-
1
-
1
-
-
-
-
-
15
-
-
2
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
643828
Fitzgerald
Further studies on the formati ...
Pseudomonas sp., Pseudomonas sp. C12B
Can. J. Microbiol.
23
483-490
1977
2
-
-
-
-
1
4
-
1
1
-
2
-
3
-
-
-
-
-
-
-
2
4
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
1
-
4
-
-
1
1
-
2
-
-
-
-
-
-
-
2
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643829
Renosto
Choline sulfokinase of Penicil ...
Penicillium chrysogenum
Arch. Biochem. Biophys.
180
416-428
1977
-
-
-
-
-
1
16
5
-
-
1
1
-
1
-
-
1
-
-
1
1
2
4
-
1
-
-
-
-
1
1
1
-
5
-
-
-
-
-
-
-
-
1
-
16
5
5
-
-
1
1
-
-
-
1
-
1
1
2
4
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
643827
Orsi
Choline sulphokinase (sulfotra ...
Aspergillus nidulans
J. Biochem.
56
81-91
1964
3
-
-
-
-
-
31
4
-
1
-
1
-
1
-
-
1
-
-
-
-
3
4
-
-
1
-
3
-
1
1
1
-
-
-
-
3
-
-
-
-
-
-
-
31
-
4
-
1
-
1
-
-
-
1
-
-
-
3
4
-
1
-
3
-
1
1
1
-
-
-
-
-
-
-