Literature summary for 2.8.2.6 extracted from

  • Renosto, F.; Segel, I.H.
    Choline sulfokinase of Penicillium chrysogenum: partial purification and kinetic mechanism (1977), Arch. Biochem. Biophys., 180, 416-428.
    View publication on PubMed

General Stability

General Stability Organism
age-inactivated enzyme can be restored to full activity by 10 min preincubation with 50 mM mercaptoethanol, prolonged incubation for 24 h results in irreversible denaturation Penicillium chrysogenum

Inhibitors

Inhibitors Comment Organism Structure
2'-AMP weak inhibitor Penicillium chrysogenum
3',5'-ADP
-
Penicillium chrysogenum
3'-AMP
-
Penicillium chrysogenum
5'-ADP
-
Penicillium chrysogenum
5'-AMP
-
Penicillium chrysogenum
Chlorocholine competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS Penicillium chrysogenum
choline substrate inhibitor Penicillium chrysogenum
choline-O-phosphate dead-end inhibitor, competitive inhibition with choline, linear mixed type inhibitor with respect to PAPS Penicillium chrysogenum
additional information 3,3-dimethylbutanol does not inhibit, enzyme is not significantly inhibited by methyl, butyl, pentyl or hexyl sulfates nor by p-nitrophenol or carnitine, EDTA is not inhibitory, sodium chloride, bromide and iodide have no effect Penicillium chrysogenum
N,N-diethylethanolamine
-
Penicillium chrysogenum
p-chloromercuribenzoate
-
Penicillium chrysogenum
Tetramethylammonium competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS Penicillium chrysogenum
thiocholine linear mixed type inhibitor with respect to PAPS Penicillium chrysogenum
Trimethylammonium competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS Penicillium chrysogenum
vinyl trimethylammonium neurine, competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS Penicillium chrysogenum
vinyl trimethylammonium bromide
-
Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
3'-phosphoadenylylsulfate pH 7.8, 37°C Penicillium chrysogenum
0.013
-
3'-phosphoadenylylsulfate pH 7.8, 37°C, N,N-dimethylaminoethanol as substrate Penicillium chrysogenum
0.8
-
N,N-Dimethylaminoethanol pH 7.8, 37°C Penicillium chrysogenum
0.017
-
choline pH 7.8, 37°C Penicillium chrysogenum
2.8
-
N,N,N-triethylaminoethanol pH 7.8, 37°C Penicillium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
gel filtration Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + choline Penicillium chrysogenum
-
adenosine 3',5'-bisphosphate + choline sulfate
-
ir

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Penicillium chrysogenum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000825
-
-
Penicillium chrysogenum

Storage Stability

Storage Stability Organism
0°C, purified enzyme relatively stable when stored in presence of 25% sucrose Penicillium chrysogenum
-20°C, 25% sucrose, loses only about 20% of its activity after 1 month Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + choline
-
Penicillium chrysogenum adenosine 3',5'-bisphosphate + choline sulfate
-
ir
3'-phosphoadenylylsulfate + choline equilibrium of the choline sulfokinase reaction lies far toward the direction of choline-O-sulfate formation, only 0.1% conversion of choline-O-sulfate to 3'-phosphoadenylylsulfate can be detected Penicillium chrysogenum adenosine 3',5'-bisphosphate + choline sulfate
-
ir
3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol 35% of the activity with choline Penicillium chrysogenum adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate
-
?
3'-phosphoadenylylsulfate + N,N,N-triethylaminoethanol 2% of the activity with choline Penicillium chrysogenum adenosine 3',5'-bisphosphate + N,N,N-triethylaminoethanol O-sulfate
-
?

Synonyms

Synonyms Comment Organism
PAPS:choline sulfotransferase
-
Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1 7.2
-
Penicillium chrysogenum

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5
-
Penicillium chrysogenum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8.8 stable for at least 15 min Penicillium chrysogenum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
9.4
-
choline-O-phosphate pH 7.8, 37°C Penicillium chrysogenum
4.1
-
3'-AMP pH 7.8, 37°C Penicillium chrysogenum
0.42
-
choline-O-phosphate pH 7.8, 37°C Penicillium chrysogenum
5.8
-
thiocholine pH 7.8, 37°C Penicillium chrysogenum
2.5 14 3',5'-ADP pH 7.8, 37°C depending on the choline concentration Penicillium chrysogenum