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Literature summary for 2.8.2.5 extracted from
- Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of a rat chondrosarcoma cell line (1999), J. Biol. Chem., 274, 2456-2463.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | stimulates | Rattus norvegicus |  |
| dithiothreitol | activation of the purified enzyme | Rattus norvegicus | |
| glutathione | reduced glutathione stimulates | Rattus norvegicus | |
| protamine | stimulates | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00027 | - |
3'-phosphoadenylylsulfate | pH 6.8, 37°C, in presence of protamine | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | enzyme is released into the cell culture medium | Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | stimulates | Rattus norvegicus | |
| Ca2+ | highest stimulatory effect, optimal concentration: 5 mM | Rattus norvegicus | |
| Fe2+ | stimulates | Rattus norvegicus | |
| Mg2+ | slightly stimulating | Rattus norvegicus | |
| Mn2+ | stimulates | Rattus norvegicus | |
| Sr2+ | stimulates | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
1 * 35000, deglycosylated protein, SDS-PAGE | Rattus norvegicus |
| 50000 | - |
about, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + chondroitin | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme contains N-linked oligosaccharides | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1920fold, to homogeneity, from the serum-free cell culture medium | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | chondrosarcoma cell line | Rattus norvegicus | - |
| chondrosarcoma cell | extracellular, secretion to the medium | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000006 | - |
purified enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme, loss of 80% activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + chondroitin | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + chondroitin sulfate C | low activity | Rattus norvegicus | adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + desulfated chondroitin sulfate A | low activity | Rattus norvegicus | adenosine 3',5'-bisphosphate + chondroitin sulfate A | - |
? | |
| 3'-phosphoadenylylsulfate + desulfated dermatan sulfate | - |
Rattus norvegicus | ? | - |
? | |
| additional information | no activity with dermatan sulfate, keratan sulfate | Rattus norvegicus | ? | - |
? | |
| additional information | chondroitin sulfate E, heparan sulfate, and completely desulfated N-resulfated heparin are no acceptor substrates | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, deglycosylated protein, SDS-PAGE | Rattus norvegicus |
| monomer | 1 * 50000-54000, nonreducing conditions, SDS-PAGE | Rattus norvegicus |
| monomer | 1 * 60000-64000, reducing conditions, SDS-PAGE | Rattus norvegicus |
| More | the deglycosylated enzyme forms dimers in solution of 70 kDa | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C4ST | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Rattus norvegicus |
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