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Literature summary for 2.8.2.5 extracted from

  • Habuchi, O.; Miyashita, N.
    Separation and characterization of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase from chick embryo cartilage (1982), Biochim. Biophys. Acta, 717, 414-421.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Gallus gallus
2-mercaptoethanol optimum concentration: 10 mM Gallus gallus
Basic proteins stimulate Gallus gallus
dithiothreitol stimulates, optimum concentration: 2.0 mM Gallus gallus
glutathione stimulates, optimum concentration: 5.0 mM Gallus gallus
histone stimulates, optimum concentration: 0.5 mg/ml Gallus gallus
lysozyme stimulates, optimum concentration: 4.0 mg/ml Gallus gallus
polyamines stimulate Gallus gallus
protamine stimulates Gallus gallus
protamine optimum concentration: 0.075 mg/ml Gallus gallus
spermidine stimulates, optimum concentration: 4.0 mM Gallus gallus
spermine stimulates Gallus gallus
spermine optimum concentration: 0.6 mM Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
3'-phosphoadenylylsulfate pH 6.8, 37°C, in presence of protamine Gallus gallus
0.8
-
3'-phosphoadenylylsulfate pH 6.8, 37°C, in addition of spermine Gallus gallus
1.4
-
3'-phosphoadenylylsulfate pH 6.8, 37°C, in addition of Mn2+ Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates Gallus gallus
Mn2+ optimum concentration: 1-10 mM Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + chondroitin Gallus gallus involved in biosynthesis of chondroitin sulfate adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cartilage embryonic Gallus gallus
-
cartilage epiphyseal Gallus gallus
-
embryo
-
Gallus gallus
-
serum in hepatitis serum 4fold more active than in normal Gallus gallus
-
synovial fluid
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + chondroitin
-
Gallus gallus adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
?
3'-phosphoadenylylsulfate + chondroitin involved in biosynthesis of chondroitin sulfate Gallus gallus adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
Gallus gallus