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Literature summary for 2.8.2.4 extracted from
- Enzymic synthesis of steroid sulphates. III. Isolation and properties of estrogen sulphotransferase of bovine adrenal glands (1967), Biochim. Biophys. Acta, 146, 493-508.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | - |
Bos taurus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | non-competitive inhibition | Bos taurus | |
| Co2+ | strong inhibition | Bos taurus | |
| additional information | only moderately sensitive to SH-blocking agents, no inhibition with EDTA up to 20 mM | Bos taurus | |
| Ni2+ | strong inhibition | Bos taurus | |
| o-Iodosobenzoate | sensitive above 10 mM | Bos taurus | |
| p-chloromercuribenzoate | sensitive above 1 mM | Bos taurus | |
| Zn2+ | strong inhibition | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
17beta-estradiol | pH 8.1, 37°C | Bos taurus | |
| 0.07 | - |
adenosine 3',5'-bisphosphate | pH 8.1, 37°C | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activated by, but no absolute requirement | Bos taurus | |
| Mg2+ | activated by, but no absolute requirement | Bos taurus | |
| Mn2+ | activated by, but no absolute requirement | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + estrone | Bos taurus | steroid metabolism | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
bovine | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, partially purified A form of enzyme loses only a small percentage of its activity on standing in ice for a period of 2 weeks, B form loses 50% of its initial activity in this time | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + 17beta-estradiol | - |
Bos taurus | adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + estriol | - |
Bos taurus | adenosine 3',5'-bisphosphate + estriol 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + estrone | - |
Bos taurus | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + estrone | steroid metabolism | Bos taurus | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + hexestrol | - |
Bos taurus | adenosine 3',5'-bisphosphate + hexestrol 4-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + stilbestrol | - |
Bos taurus | adenosine 3',5'-bisphosphate + stilbestrol 4-sulfate | - |
r | |
| additional information | simple phenols, 2-naphthylamine and 3beta-hydroxysteroids are not sulfated, dienestrol is no substrate, dehydroepiandrosterone, phenol, p-nitrophenol, alpha-naphthol, beta-naphthol, beta-naphthylamine and 17 beta-estradiol-3-methyl ether fail to act as substrates | Bos taurus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
ADP | pH 8.1, 37°C | Bos taurus | |
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Release 2023.1 (January 2023)
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