Literature summary for 2.8.2.34 extracted from

Barnes, S.; Burhol, P.G.; Zander, R.; Haggstrom, G.; Settine, R.L.; Hirschowitz, B.I.
Enzymic sulfation of glycochenodeoxycholic acid by tissue fractions from adult hamsters (1979), J. Lipid Res., 20, 952-959.

Search for more literature for 2.8.2.34

Inhibitors

Inhibitors	Comment	Organism	Structure
chenodeoxycholate	competitive, 50% inhibition at about 0.1 mM	Mesocricetus auratus
glycolithocholate	competitive, 50% inhibition at 0.04 mM	Mesocricetus auratus
ursodeoxycholate	competitive, 50% inhibition at about 0.1 mM	Mesocricetus auratus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Mesocricetus auratus
0.00023	-	glycochenodeoxycholate	male hamster, pH 7.0, 37°C, physiological substrate concentration	Mesocricetus auratus
0.00142	-	glycochenodeoxycholate	female hamster, pH 7.0, 37°C, physiological substrate concentration	Mesocricetus auratus
0.079	-	glycochenodeoxycholate	female hamster, pH 7.0, 37°C, saturating substrate concentration	Mesocricetus auratus
0.317	-	glycochenodeoxycholate	male hamster, pH 7.0, 37°C, saturating substrate concentration	Mesocricetus auratus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Mesocricetus auratus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity, best at 2.5 mM	Mesocricetus auratus
additional information	activity is independent of ionic strength in the range of 25-125 mM	Mesocricetus auratus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3'-phosphoadenylyl 5'-phosphosulfate + glycochenodeoxycholate	Mesocricetus auratus	-	adenosine 3',5'-bisphosphate + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mesocricetus auratus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
adrenal gland	-	Mesocricetus auratus	-
intestine	proximal	Mesocricetus auratus	-
liver	-	Mesocricetus auratus	-
additional information	no activity in herat, spleen, skeletal muscle, brain, distal small intestine, and large intestine, activity is higher in femal than in male hamsters	Mesocricetus auratus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000003	-	adrenal gland supernatant fraction	Mesocricetus auratus
0.000004	-	proximal intestine supernatant fraction, female hamster	Mesocricetus auratus
0.000005	-	proximal intestine supernatant fraction, male hamster	Mesocricetus auratus
0.000017	-	liver supernatant fraction, male hamster	Mesocricetus auratus
0.000062	-	liver supernatant fraction, female hamster	Mesocricetus auratus

Storage Stability

Storage Stability	Organism
-20°C, hepatic and intestinal supernatant fractions, completely stable for at least 6 months	Mesocricetus auratus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl 5'-phosphosulfate + glycochenodeoxycholate	-	Mesocricetus auratus	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylyl 5'-phosphosulfate + glycochenodeoxycholate	-	Mesocricetus auratus	adenosine 3',5'-bisphosphate + ?	product identification	?

Synonyms

Synonyms	Comment	Organism
BAST	-	Mesocricetus auratus
bile acid:PAPS:sulfotransferase	-	Mesocricetus auratus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Mesocricetus auratus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Mesocricetus auratus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	-	Mesocricetus auratus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.018	-	glycolithocholate	pH 7.0, 37°C	Mesocricetus auratus
0.071	-	chenodeoxycholate	pH 7.0, 37°C	Mesocricetus auratus
0.083	-	ursodeoxycholate	pH 7.0, 37°C	Mesocricetus auratus

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Release 2023.1 (January 2023)