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Literature summary for 2.8.2.34 extracted from

  • Takikawa, H.; Stolz, A.; Kaplowitz, N.
    Purification of a 32.5 kDa monomeric sulfotransferase from rat liver with activity for bile acids and phenolic steroids (1986), FEBS Lett., 207, 193-197.
    View publication on PubMed
Search for more literature for 2.8.2.34

General Stability

General Stability Organism
20% glycerol stabilizes Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male sprague-dawley rats
-

Purification (Commentary)

Purification (Comment) Organism
from liver, by gel filtration, affinity chromatography, chromatofocusing, and hydroxyapatite chromatography, to homogeneity Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000094
-
 purified enzyme, substrate glycolithocholate Rattus norvegicus
0.000831
-
 purified enzyme, substrate estradiol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl 5'-phosphosulfate + estradiol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylyl 5'-phosphosulfate + estradiol
-
 Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylyl 5'-phosphosulfate + glycolithocholate
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
-
 ?
3'-phosphoadenylyl 5'-phosphosulfate + glycolithocholate
-
 Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
-
 ?
3'-phosphoadenylyl 5'-phosphosulfate + taurolithocholate
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + taurolithocholate 3-sulfate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus chromatofocusing
-
 5.1