General Stability | Organism |
---|---|
freeze-thawing reduces activity, enzyme has very low stability at 4°C especially during concentration procedures | Heterodontus portusjacksoni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 3',5'-diphosphate | competitive inhibition | Heterodontus portusjacksoni | |
EDTA | 50 mM, 89% inhibition | Heterodontus portusjacksoni | |
iodoacetic acid | 0.1 mM, 29% inhibition | Heterodontus portusjacksoni | |
NaN3 | 0.1 mM, 39% inhibition | Heterodontus portusjacksoni | |
p-chloromercuribenzoate | 0.1 mM, 38% inhibition | Heterodontus portusjacksoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
(24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | room temperature, pH 6.5 | Heterodontus portusjacksoni | |
0.0044 | - |
3'-phosphoadenosine 5'-phosphosulfate | room temperature, pH 6.5 | Heterodontus portusjacksoni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Heterodontus portusjacksoni | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | Heterodontus portusjacksoni | trivial name 5beta-scymnol | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Heterodontus portusjacksoni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
hydroxyapatite, DEAE-Sephacel, Sephadex G-100, partially purified | Heterodontus portusjacksoni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Heterodontus portusjacksoni | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000002 | - |
- |
Heterodontus portusjacksoni |
Storage Stability | Organism |
---|---|
-80°C, at least 12 months, no loss of activity | Heterodontus portusjacksoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol | trivial name 5beta-scymnol | Heterodontus portusjacksoni | adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate | trivial name 5beta-scymnol sulfate | ? | |
3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol | - |
Heterodontus portusjacksoni | adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate | - |
? | |
3'-phosphoadenosine 5'-phosphosulfate + testosterone | - |
Heterodontus portusjacksoni | adenosine 3',5'-diphosphate + testosterone sulfate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Heterodontus portusjacksoni |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | - |
Heterodontus portusjacksoni |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00037 | - |
adenosine 3',5'-diphosphate | vs. 3'-phosphoadenosine 5'-phosphosulfate | Heterodontus portusjacksoni | |
0.00084 | - |
adenosine 3',5'-diphosphate | vs. 5beta-scymnol | Heterodontus portusjacksoni |